摘要
The functional analysis of dr1127,a novel gene in Deinococcus radiodurans was performed in this pa-per. The dr1127 gene was found occasionally in our microarray and 2-DE gel experiments. Mutation of the dr1127 gene decreased the γ-radiation and H2O2 resistance of D. radiodurans,and weakened the scavenging abilities of cell extracts for free radicals (superoxide anion,hydrogen peroxide,and hy-droxyl radical). Further oxidative damage assays demonstrated that the purified DR1127 protein of D. radiodurans could bind to double stranded DNA in vitro and protect DNA from oxidative damage in this way. These results suggest that the dr1127 gene is an important gene that can maintain γ-radiation and oxidative resistance in D. radiodurans and may take part in the oxidative stress process.
The functional analysis of dr1127, a novel gene in Deinococcus radiodurans was performed in this paper. The dr1127 gene was found occasionally in our microarray and 2-DE gel experiments. Mutation of the dr1127 gene decreased the y-radiation and H202 resistance of D. radiodurans, and weakened the scavenging abilities of cell extracts for free radicals (superoxide anion, hydrogen peroxide, and hydroxyl radical). Further oxidative damage assays demonstrated that the purified DR1127 protein of D. radiodurans could bind to double stranded DNA in vitro and protect DNA from oxidative damage in this way. These results suggest that the dr1127 gene is an important gene that can maintain γ-radiation and oxidative resistance in D. radiodurans and may take part in the oxidative stress process.
基金
the National Basic Research Program of China (Grant No. 2004 CB 19604)
Grant for Distinguished Young Scientist of China (Grant No. 30425038)
National Natural Science Foundation of China (Grant No. 30330020)
关键词
dr1127
异常基因
氧化损伤
基因突变
dr1127, Deinococcus radiodurans, oxidative damage, dsDNA binding