强肌健力饮对肾阳虚大鼠CRH、ACTH、Cor水平的影响

Effects of Qiangji Jianli Yin on the Hypothalamus CRH Contents and Plasma ACTH,Cortisol Levels in Rat Models of Kidney-Yang Deficiency Syndrome

目的：观察强肌健力饮对肾阳虚大鼠下丘脑组织中促肾上腺皮质激素释放激素（CRH）及血浆促肾上腺皮质激素（ACTH）、皮质醇（Cor）水平的影响,进一步探讨该方对中医肾阳虚证的防治机理。方法：将大鼠分为正常对照组、肾阳虚模型组、强肌健力饮低、中、高剂量组、右归丸阳性对照组,采用氢化可的松制备肾阳虚大鼠模型。观察动物的一般状态及其胸腺和肾上腺指数,采用放射免疫分析检测CRH、ACTH、Cor的含量。结果：①肾阳虚模型组大鼠体重及胸腺指数、肾上腺指数明显低于正常对照组（P〈0.01）,CRH、ACTH、Cor含量均比正常对照组显著降低（P〈0.01）。②强肌健力饮各剂量组CRH、ACTH、Cor含量均比肾阳虚模型组显著升高（P〈0.05~0.01）。结论：肾阳虚时下丘脑-垂体-肾上腺轴合成、分泌和调控功能低下,而强肌健力饮能够修复该轴功能的损伤,表明该方药具有下丘脑、垂体、肾上腺轴多层次的调节作用。

Objective To investigate the effects of qiangji jianli yin on hypothalamus CRH contents and plasma ACTH,Cortisol levels in rat models with kidney - yang deficiency syndrome. Methods Hat models of kidney - yang deficiency syndrome were prepared with intramuscular injuection of hydrocortisone and divided into 5 groups ： （1） no further treatment, n = 13 （2） treated with high dosage of qiangji jianli Tin, n = 12 （3） treated with medium dosage of qiangji jianli yin, n = 12 （4） treated with low dosage of qiangji jianli yin n = 12, （5） treated with yougui wan, n = 12. Ten rats injuected with intramuscular distilled water only served as controls. The animals were sacrificied 14 days later and the hypothalamus CBH contents as well as plasma AOM and cortisol levels were measured with RIA. The thymus gland weight index and the adrenal gland index were calculated. Results （1） The hypothalmus CRH contents and plasma ACTH, cortisol levels were significantly lower （ P 〈 0.01 ） in the rat models of kidney - yang deficiency syndrome without any treatment thas those in controls rats; the thymus and adrenal gland weight index were significantly decreased too （P 〈0.01 ）. （2） The CRH conteats and ACTH, cortisol levels in all the three group of rat model treated with different dosage of qiangji jianli yin were significantly higher than those in the models without any treatment （P 〈 0.05 - 0.01 ）. Conclusion In rat models of kidney - yang deficiency syndrome, dysfunction of the hypothalamus - pituitary - adrenal axis （HPAA） led to decreased secretion of related hormanes. The HPAA function might be partially restored with administation of qiangji jianli Tin.