严重创伤患者外周血sCD14水平变化及临床意义

Changes of Plasma Soluble CD14 (sCD14) Contents in Patients with Severe Trauma

目的:观察重度创伤患者sCD14水平的变化与病情的关系。方法:ELISA法检测外周血单个核细胞体外培养上清液和外周血血浆中sCD14含量和五分类法检测外周血单核细胞数量和比率。结果:22例重度创伤患者手术24h后外周静脉血单个核细胞培养上清液中的sCD14水平(4.50ng/ml)较健康对照组(1.17ng/ml)明显升高(P<0.05),而手术后48h的sCD14有明显降低(1.15ng/ml),血浆中的sCD14在手术前较高(11.85ng/ml),同时外周血单核细胞数量和比率不同程度升高。结论:在重度创伤患者检测sCD14和外周血单核细胞的变化有一定的临床意义。

Objective To study the changes of serum soluble CD14 （sCD14） contents in patients with severe trauma. Methods Plasma sCD14 contents as well as sCD14 contents in mononuelear cell culture supernatant （ in vitro） were measured with ELISA in 22 patients with severe trauma and 30 controls. Results In the patients with severe trauma, level of sCD14 in supematant liquid of peripheral blood mononuclear cells culture in vitro 24h after operation （4.50ng/ml）was obviously higher than those in controls（ 1.17 ng/ml, P 〈 0.05 ）, the level of sCD14 was decreased（ 1.15 ng/ml） at 48h after operation. Before operation higher plasma level（ 11. 85 ng/ml） of sCD14 was noted （ vs controls, P 〈 0.05 ）. Mean while, peripheral blood mononuclear cell count were increased. Conclusion Changes of sCD14 contents （ both in plasma and mononuclear cell culture） post - operatively in patients with severe trauma might be of prognostic value.