U-1分解草酸的检测及其降低草酸毒性的研究

Study on the decomposing ability of U-1 to oxalic acid and its mechanisms in decreasing the toxicity of oxalic acid(OA)

草酸是油菜菌核病病菌(Sclerotinia sclerotiorum)致病的重要因子。以实验室分离得到的草酸分解菌U-1为材料,研究U-1分解草酸的能力及其降低草酸对油菜毒性的作用机理。检测U-1分解草酸的能力,即以草酸、U-1+草酸分别培养24 h后,利用高效液相色谱仪检测草酸的含量;研究U-1抑制草酸对油菜毒害的作用机理,即分别以清水、U-1、草酸、U-1+草酸处理油菜叶片,检测不同处理对油菜叶片中防卫反应0相关酶(PAL酶、PPO酶和PO酶)变化的影响。研究结果表明,与草酸单独处理组相比,U-1+草酸处理组草酸含量显著降低;U-1可以显著抑制草酸对油菜中PAL酶、PPO酶和PO酶活性的作用。

Oxalic acid（OA） is an important factor during Sclerotinia sclerotiorum effects.Using OA-decomposing bacterium isolated by our laboratory as materials,we detected the decomposing ability of U-1 to OA and its mechanisms in decreasing the toxicity of OA to rapes.Firstly,the OA-decomposing ability of U-1 was tested by culturing OA U-1＋OA respectively for 24 h,and then the content of OA by HPLC was tested.Secondly,we studied the mechanisms of U-1 in restraining the toxicity of OA to rapes by treating rapes by with water,U-1,OA,U-1＋OA respectively,the activities of defense-related enzymes involving phenylalanine ammonia lyase（PAL）,peroxidase（PO） and polyphenoloxidase（PPO） were determined.The results showed that,after U-1 and OA were co-cultured for 24 h,36 h,48 h and 60 h,the OA was significantly decomposed by U-1;U-1 significantly restraining the effects of OA to enzymes of PPO,PAL and PO in rapes.