荧光法研究阳离子水溶性聚芴与牛血清白蛋白的相互作用

Study on the Interaction between Water-soluble Polyfluorene and Bovin Serum Albumin by Fluorescence Method

用荧光光谱法研究了阳离子水溶性聚芴:聚[9,9-二(3′-(N,N-二甲基)-N-乙基铵)丙基)-2,7-芴)-alt-1,4-亚苯基]二溴化物(PF)与生物大分子牛血清白蛋白(BSA)之间的相互作用,并进一步研究了酸度和离子强度对二者相互作用的影响。结果表明,极微量的BSA便会使PF的荧光产生可分辨的降低,改变蛋白质BSA的表面电荷,BSA对PF的猝灭效率也发生较大变化;而PF可猝灭BSA的荧光,同时自身的荧光增强并存在一个等发射点,说明BSA和PF之间存在相互作用,二者之间发生了能量转移。

The interaction between the conjugated polymer Poly[（9,9-bis（3′-（N, N-dimethyl）-N-ethylammonium）- propyl）-2,7-fluorene）-alt-1,4-phenylene] Dibromide（PF） and biomolecule bovine serum albumin （BSA）, as well as the influence of the acidity and the ion intensity on the interaction between them, were studied by fluorescence spectroscopic technique. The results showed that the addition of infinitesimal BSA could decrease the fluorescence intensity of PF. When the surface charge of BSA was changed, the quenching efficiency was also altered. Meanwhile, the fluorescence of BSA can be quenched with the addition of PF and the fluorescence intensity of PF itself increased. The spectra displayed iso emission point at 394.5nm and it demonstrated that there existed the interaction and energy transfer between PF and BSA.