应用阳离子脂质体介导内皮抑素和（或）p53基因治疗Lewis肺癌小鼠的实验研究

SA cationic iiposomes complexed to plasmids encoding endostatin and/or p53 inhibit Lewis lung cancer in mice model

目的 观察SA阳离子脂质体介导内皮抑素基因和（或）p53基因对Lewis肺癌小鼠移植瘤生长、转移的抑制作用。方法 取Lewis肺癌细胞悬液建立C57BL/6j小黑鼠肺癌动物模型后,选择40只随机分成5组,每组8只,分别为空白对照组、实验对照组、p53治疗组、pEnd治疗组、pEnd＋p53联合治疗组。小鼠成瘤后,瘤内注射SA阳离子脂质体介导的内皮抑素基因和（或）p53基因,每周2次,共6周。观察瘤体大小变化、小鼠营养状况、生存期等。结果 各治疗组均能抑制肿瘤生长及肺内转移,与对照组比较有统计学意义（P〈0.01）,小鼠活动能力、饮食、对外界刺激反应能力等均优于对照组。结论 应用SA阳离子脂质体介导内皮抑素基因和（或）p53基因瘤内注射可有效地抑制Lewis肺癌移植瘤的生长、转移,小鼠生存期明显延长于对照组。内皮抑素基因与p53基因联合未发现协同作用。

Objective To evaluate the inhibitory effection of SA cationic liposomes complexed plasmids encoding endostatin and/or p53 on Lewis lung cancer in mice model. Methods Establish Lewis lung cancer mice model, 40 of them were divided into five groups randomly, each group had six mice, administrated intratumorally SA cationic liposome complexed plamids encoding endostatin and p53, twice a week,for six weeks in all. The function of inhibiting tumor and antiangiogenesis were evaluated according to the size change of the tumor, the activity, food taking, nourishment, the existent time of the mice and metastasis in lung. Results The treatment groups could inhibit the growth and me tumor. Comparing with control groups, the treatment groups showed significance in metastasis in lung and survival period of the mice. Conclusions Intratumoral a liposome complexed plamids encoding endostatin and p53 can powerfully inhibit the implanted Lewis lung cancer in mice model;endostatin and p53 show no collaborati tastasis of the implanted tumor size（ P 〈0.01）, dministration of cationic growth and metastasis of ve efficacy.