广州地区诱导痰细胞学正常参考值的建立

Establishment of reference values for cellularity in induced sputum of healthy adults in Guangzhou

目的 建立广州地区正常人诱导痰细胞总数及分类的正常范围。方法 对117例正常非吸烟的自愿者进行高渗盐水雾化痰诱导,并对诱导痰液中细胞总数及细胞分类进行检测和统计分析。结果 108例自愿者（男60例,女48例）成功诱导出痰液,诱导痰成功率为94.2%,细胞总数和分类结果的x^-±2SD、中位数（四分位数间距）分别为,细胞总数：2.32±3.54、2.1（1.6）%,中性粒细胞：46.9±52.3、42.9（41.2）%,巨噬细胞：51.2±52.3、55.8（41.3）%,嗜酸粒细胞：0.4±1.6、0.0（0.5）%,淋巴细胞：1.4±2.8、1.0（2.0）%。正常男女对照的各种参考值范围结果无统计学差异。结论 本研究初步建立了本地区诱导痰细胞总数及细胞分类的正常参考值范围,在研究气道炎症的特征和相应患者的诊疗等方面具有一定的意义。

Objective To establish reference values for total and differential cell counts in induced sputum of healthy adults in Guangzhou. Methods The total and differential cell counts were examined in induced sputum from samples of a total of 117 healthy nonsmoking adult volunteers. Results Sputum induction produced an adequate sample in 108 subjects （60 males, 48 females）, the achievement ratio was 94.2%. The mean± 2SD,median （IQR） of total cell count was 2.32±3.54,2.1（1.6）%. The proportions of neutrophils were 46.9± 52.3,42.9 （41.2）%, macrophages 51.2 ± 52.3,55.8 （41.3） %, eosinophils 0.4 ± 1.6,0.0 （0.5）% and lymphocytes 1.4±2, 8,1.0 （2.0）%, respectively. There were no significant differences in the total and differential cell counts between male and female（ P 〉0.05）. Conclusions The reference values were established for total and differential cell counts in induced sputum of healthy adults in Guangzhou. We propose that these data be used in research on pathogenesis, diagnosis and treatment of the patients with airway inflammation.