Ⅱ-型拟除虫菊酯对雄性小鼠中枢神经系统氧化应激的影响

EFFECTS OF II-PYRETHRINS ON OXIDATIVE STRESS OF CENTRAL NERVOUS SYSTEM OF MICE

[目的]探索II-型拟除虫菊酯类农药对小鼠脑的氧化损伤作用及其毒理作用机制。[方法]将50只雄性昆明系小鼠随机分为5组(每组10只),即溴氰菊酯(DM)1.8mg/kg组、3.6mg/kg组;氯氰菊酯(CP)4mg/kg组、8mg/kg组及色拉油对照组;连续1个月经口灌胃染毒后,检测DM、CP对小鼠脑中丙二醛(MDA)、羟自由基(-OH)、总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(CSH-PX)活性的影响。[结果]DM1.8mg/kg组和CP4mg/kg组小鼠脑内丙二醛(MDA)和谷胱甘肽过氧化物酶(CSH-PX)的含量与对照组比较差异有统计学意义;DM3.6mg/kg组和CP8mg/kg组小鼠脑内检测的四种指标与对照组相比均有统计学意义。[结论]II-型拟除虫菊酯类农药可引起小鼠脑内MDA、-OH、T-AOC、CSH-PX活性的改变,导致机体抗氧化能力下降,使之对外来化学物氧化损伤作用的敏感性增高。

[Objective] To study oxidative damage of II- Pyrethrins on brains of mice and the mechanism of its toxic effect. [Methods] A total of 50 Kunming mice were randomly divided into 5 groups： （1） deltamethrin （DM） 1.8 mg/kg group; （2）deltamethrin （DM） 3.6mg/kg group ;（3）cypermethrin （CP）4 mg/kg group; （4） cypermethrin （CP） 1.8 kg/kg group （5） normal saline group. Different doses of DM and CP in corn oil were administered to mouse by, gavage once daily month. The content of malondialdehyde （MDA） the activities of-OH, T-AOC and glutathione peroxidase （GSH-Px） in mice brains were determined. [Results] The contents of MDA and GSH-px in DM 1.8mg/kg group and CP4 mg/kg group Were significant different from the control group. And the indexes of MDA,-OH, T-AOC and GSH-px in DM3.6mg/kg group and CP8mg/kg group were obviously different from the Control group. [Conclusions] II- Pyrethrins might cause the Changes 0f MDA.-OH, T-AOC and GSH-px in brains of mice, further decrease the antioxidation of the body, finally lead to the increase of its sensitivity to oxidative damage of ectogenic chemical materials.