HPLC法同时测定肠肛舒口服液中芍药苷和黄芩苷的含量

Simultaneous Determination of Peoniflorin and Baicalin in Changgangshu Oral Liquid by HPLC

目的:建立以高效液相色谱法同时测定肠肛舒口服液中芍药苷和黄芩苷含量的方法。方法:色谱柱为Ultimate XB-C18(250mm×4.6mm,5μm),流动相为甲醇-水-醋酸(35:65:1),流速为1.0mL.min-1,检测波长为244nm。结果:芍药苷和黄芩苷分别检测浓度在0.025～0.25mg.mL-1范围内与峰面积积分值呈良好线性关系,相关系数(r)分别为0.9997和1.0000;平均回收率分别为98.4%和97.0%,RSD分别为1.43%和1.20%(n=9)。结论:本法可同时测定肠肛舒口服液中芍药苷和黄芩苷的含量,方法简便、准确,可为制定肠肛舒口服液质量标准及产品质量控制提供依据。

OBJECTIVE： To establish a HPLC method for the simuhaneous determination of Peoniflorin and Baicalin in Changgangshu oral liquid. METHODS： The determination was performed on tlhimate XB-C18（250mm×4.6mm, 5/,m） with mobile phase consisted of methanol-water-acetic acid（35：65：1） at a flow rate of 1.0mL·min^-1. The detection wavelength was set at 244nm. RESULTS： The linear ranges of Peoni florin and Baiealin were 0. 025- 0.25mg·mL^-1（ r = 0. 999 7 vs. 1. 000 0）, with average recovery rates at 98.4% vs.97.0%, RSD = 1.43% vs. 1.20% （ n = 9） . CONCLUSION：The method was proved to be simple and accurate, and it can be used to determination Peoniflorin and Baicalin simultaneously in Changgangshu oral liquid,furthermore,it provides basis for the setting of quality specification and quality control of Changgangshu oral liquid.