他扎罗汀诱导前后角质形成细胞TIG 3 mRNA表达

Effects of Tazarotene on the Expression of Tazarotene-induced Gene 3 mRNA in Normal Human Keratinocytes

目的研究他扎罗汀对培养的正常人角质形成细胞增殖和他扎罗汀诱导基因3(TIG3)mRNA表达的影响。方法用0.1和1.0μmol/L他扎罗汀处理正常人角质形成细胞24h后,用MTT法、RT-PCR和实时定量RT-PCR(TaqMan探针)法分别检测细胞增殖和TIG3mRNA表达的改变。结果他扎罗汀可抑制角质形成的增殖和诱导TIG3mRNA的表达。0.1和1.0μmol/L他扎罗汀处理角质形成细胞后,分别使角质形成细胞增殖抑制5.0%和11.1%,使TIG3mRNA增加到正常对照组的2.1倍和2.8倍。结论他扎罗汀可抑制角质形成细胞的增殖和上调TIG3mRNA的表达。

Objective To investigate the alteration of cell proliferation and tazarotene-induced gene 3 （TIG3） mRNA expression in cultured normal human keratinocytes after tazarotene treatment. Methods After 24-hour incubation with 0. 1 and 1 μmol/L tazarotene of normal human keratinocytes, the proliferation potency of the cells was detected by MTT colorimetric assay and the expression of TIG3 mRNA was examined by reverse transcription polymerase chain reaction （RT-PCR） and real-time quantitative RT-PCR （TaqMan）. Results After treatment with 0.1 and 1 μmol/L tazarotene,keratinocytes proliferation was inhibited by 5.0% and 11.1%, and TIG3 mRNA expression was induced by 2.1- and 2.8-fold, respectively. Conclusion Tazarotene can in- hibit keratinocytes proliferation and elevate TIG3 mRNA expression.