慢性特发性荨麻疹患者外周血单一核细胞干扰素-γ受体mRNA表达

Expression of IFN-γ Receptor mRNA in PBMC From the Patients with Chronic Idiopathic Urticaria

目的探讨慢性特发性荨麻疹(CIU)患者外周血单一核细胞(PBMC)干扰素-γ受体(IFN-γR)mRNA表达情况。方法收集45例CIU患者,38例正常人作为对照,采集PBMC提取RNA,应用逆转录聚合酶链扩增(RT-PCR)法检测IFNR mRNA表达水平。结果CIU患者PBMC中Th1细胞因子受体IFN-γR mRNA表达水平(0.636±0.194),高于健康对照组(0.540±0.184)(t=2.315,P(0.05)。结论CIU患者PBMC中IFN-γR的mRNA表达增高,可能与该病发病机制有关。

Objective To investigate the expression levels of interferon-gamma receptor （IFN-γR） mRNA in peripheral blood mononuclear cells （PBMCs） from patients with chronic idiopathic urticaria （CIU）. Methods PBMCs were separated from the peripheral blood specimens of 45 patients with CIU and 38 healthy controls. Reverse transcription - polymerase chain reaction （RT-PCR） technique was applied to analyze the expression of IFN-γR in PBMC. Results The mRNA expression level of IFN-γR in CIU was significantly higher than that of healthy controls. Conclusion IFN-γR mRNA expression was significantly increased, which suggest that IFN-γR might be involved in the pathogenesis of CIU.