超低温保存时间与抗冻剂浓度对真鲷精子质量的影响

The effect of storage time and cryoprotectant concentration on sperm quality of red seabream(Pagrus major)

研究了不同浓度的抗冻剂二甲基亚砜（DMSO）和不同保存时间对真鲷（Pagrus major）冻精质量的影响。试验结果表明，DMSO体积分数和保存时间对冻精的受精率、孵化率影响显著。15％二甲基亚砜作为抗冻剂保存10～60d的冻精受精率、孵化率与鲜精无差异，而保存360d的冻精受精率、孵化率开始出现下降。12％，18％，21％DMSO保存60d的冻精受精率差异不显著，均高于90％，而对于保存360d的冻精受精率、孵化率均显著地降低。

The present study focused on the effects of the storage time and cryoprotectant concentration on the post-thaw sperm quality in red seabream （Pagrus major）. The results demonstrate that cryoprotectant concentration and storage time could significantly influence the fertilization and hatching rates of post-thaw sperm. The fertilization capability of the posvthaw sperm cryopreserved with 15% DMSO for 360 d began to decrease; however, no significant difference was found among 10～60 d cryopreserved sperm. No significant differences were found among 12%～ 21 0% DMSO cryoopreserved sperm for 60 d in fertilization capacity, and the fertilization rate was higher than 90%; however, the fertilization capacities of 12%,18%, 21% DMSO cryopreserved for 360 d were reduced significantly. The effects of the storage time, and cryoprotectant concentration,and their interaction still need further study.