单胺氧化酶A基因多态性与精神分裂症的关联研究

Astudy of the polymorphisms of monoamine oxidase A genes with schizophrenia

目的探讨河南地区汉族人群单胺氧化酶A（monoamine oxidase A，MAOA）基因多态性与精神分裂症的关系。方法参照CCMD-3诊断标准，选取212例精神分裂症患者与168名正常对照，应用聚合酶链反应及限制性片段长度多态性技术检测MAOA基因多态性，采用病例一对照的关联分析方法对精神分裂症患者及正常对照的基因型和等位基因频率进行分析。结果（1）MAOA基因的基因型在患者组和对照组中均符合Hardy-Weinberg平衡定律（X^2=0．618，dr：2，P〉0、05；X^2=3．173，df=2，P〉0．05）。（2）MAOA基因的基因型和等位基因频率在患者组与对照组间的分布差异无统计学意义（P〉0．05）。（3）按性别分组，男性患者组中CT基因型分布频率显著高于男性对照组（X^2=7．654，P=0．022）。（4）MAOA基因的基因型和等位基因频率在家族史阴、阳性间的分布差异无统计学意义（P〉0．05）。结论没有发现MAOA基因多态性与汉族精神分裂症的发病有关联，但对性别发病有影响，基因型CT可能是男性精神分裂症发病的易感因素。

Objective To investigate the relationship between monoamine oxidase A （ MAOA ） gene polymorphisms and schizophrenia in a Chinese Han population. Methods Two hundred and twelve schizophrenic patients and 168 healthy controls were recruited according to CCMD-3. The polymorphisms of MAOA gene were determined with the polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） technique. The case-control association analysis was adopted to analyze the frequencies of genotype and allele in schizophrenic patients and controls. Results （1） The genotypes of MAOA gene were consistent with Hardy-Weinberg equilibrium in patient group and control group （X^2 = 0.618, df= 2, P 〉 0.05;X^2 = 3.173, df= 2, P 〉 0.05）. （2） qlae distributions of genotypes or alleles of MAOA genes had no significant difference between patient group and control group（ P 〉 0.05）. （3）Divided by sex, the frequency of CT genotype in male patients was higher than that in male controls （ X^2 = 7. 654, P = 0.022）. （4） There were no significant differences of genotypic and allelic distribution in MAOA genes between schizophrenic patients with positive family history and schizophrenic patients with negative family history and among different clinical subtypes in schizophrenic patients （ P 〉 0.05）. Conclusion No association between MAOA gene and schizophrenia is found in Chinose Han population, but CT genotype is likely to be a susceptibile factor of male schizophrenia.