HLA-DRBl＊04等位基因多态性与乙型肝炎病毒感染结局关联分析

Association of polymorphism on HLA-DRBI ＊ 04 alleles with outcome of hepatitis B virus infection

目的从分子遗传学角度探讨乙型肝炎病毒（hepatitis B virus，HBv）感染结局与GLA—DRBl＊04等位基因的相关性。方法采用聚合酶链反应一序列特异性引物（polymerase chain reaction-sequence specific primers，PCR-SSP）技术检测HLA-DRBl＊04等位基因，并比较106例无症状HBV携带者（HBV携带组），93例慢性乙型肝炎患者、77例乙肝肝硬化者、102例HBV感染后自然恢复者（对照组）HIA-DRBl＊04等位基因频率及HBV不同复制状态下HLA-DRBl＊04等位基因频率。结果HBV携带组、慢性乙肝组、乙肝肝硬化组HLA-DRBl＊04等位基因频率高于对照组（分别为25．94％、26．34％、27．92％和14．22％，P〈0．01）；HLA—DRBl＊0401基因频率高于对照组（分别为20．91％、24．49％、22．09％和8．62％，P均〈0．05）；HLA-DRBl＊0405基因频率较对照组低（3．64％、2．04％、3．49％和15．52％，P〈0．01、0．01、0．05）。各病例组之间HLA—DRBl＊04等位基因频率差异无统计学意义（P〉0．05）。HBV不同复制状态HLA-DRBl＊04等位基因频率差异无统计学意义（P〉O．05）。结论HLA-DRBl＊04是决定HBV感染结局的因素之一，但不影响HBV在体内复制。

Objective To investigate the relation between the alleles of HLA-DRB1 ＊ 04 and outcome of HBV infection. Methods The alleles of HLA-DRB1 ＊ 04 were detected by polymerase chain reaction-sequence specific primer （PCR-SSP）. The frequency of allele of HLA-DRB1 ＊ 04 in four groups[ 106 asymptomatic HBsAg carriers （group ASC） ,93 chronic hepatitis B patients （group CHB） ,77 patients with hepatitis B cirrhosis and 102 cases of spontaneous recovery after HBV infection （control group） ] were studied, and the frequency of that in different replication of HBV was also studied. Results The frequency of allele of HLA-DRB1 ＊ 04 in groups ASC, CHB and hepatitis B cirrhosis was markedly higher than that of control group （25.94%, 26.34%, 27.92% respectively versus 14.22%, P 〈 0.01）; the frequency of HLA-DRB1 ＊ 0401 in groups ASC, CHB and hepatitis B cirrhosis was also higher than that of control group （20.91%, 24.49%, 22.09% respectively versus 8.62%, P〈0.05, P〈0.01,P〈0.05 respectively）; the frequency of HLA-DRB1 ＊ 0405 in groups ASC, CHB and hepatitis B cirrhosis was lower than that of control group （3.64% ,2.04% ,3.49% respectively versus 15.52%, P 〈 0.01, P 〈 0.01, P 〈 0.05 respectively ） . There was no statistical significance in the allele frequency of HLA-DRB1 ＊ 04 among groups ASC, CHB and hepatitis B cirrhosis（P〉 0.05）, and the same result was observed in different replication of HBV （ P 〉0. 05）. Conclusio HLA-DRB1 ＊ 04 gene is one of the factors which determine the outcomes of HBV infection, while it has no influence on HBV replication.