系统性红斑狼疮细胞与其病情活动性的相关性研究

Research on the correlation between lupus erythematosus cell and diseases activity in patients with systemic lupus erythematosus

目的观察系统性红斑狼疮(SLE)患者外周血狼疮细胞(LEC)形态的变化,探讨其与SLE疾病活动性的关系。方法采用经典改良血块法观察了50例SLE疾病活动期和30例SLE非活动期患者外周血LEC形态,同时与血清自身抗体、补体及SLE疾病活动指数(SLEDAI)对比研究。结果特殊形态的LEC与自身抗体的抗dsDNA抗体和抗核小体抗体(AnuA)(r=0.588,P=0.056;r=0.759,P=0.135),补体C3和C4(r=-0.648,P=0.058;r=-0.589,P=0.057)及SLEDAI(r=0.686,P<0.05)具有明显相关性。SLE活动期组特殊型LEC、自身抗体和补体与SLE非活动期组差异有统计学意义(P<0.01)。当自身抗体和补体与SLEDAI无相关性时,特殊型LEC仍具有良好的相关性(r=0.786,P<0.05)。结论特殊型LEC与自身抗体、补体及SLEDAI有明显的相关性,可作为判断SLE疾病活动性的独特指标。

Objective To observe the morphologic changes of peripheral blood lupus cell in patients with systemic lupus erythematosus and investigate its relationship with disease activity in systemic lupus erythematosus. Methods Modified classical blood clotting method to observe the morphological changes of peripheral blood lupus cells in 80 cases with systemic lupus erythematosus. Fifty cases were in active stage, and 30 cases were in stable stage. Comparison of serum autoantibody, complement and SLEDAI were also carried out. Results There was significant association between lupus cells in special morphous and autoantibody, such as anti-double-stranded DNA antibody, anti-nucleosome antibodies, complement C3,C4 and SLEDAI （r= 0.588, P=0.056; r=0.759, P=0.135; r=-0.6d8, P=0.058; r=-0.589, P=0.057; r=0.686, P〈0.05）. There was significant difference in special morphous LEC, autoantibody and complement between the active disease group and the stable patients （P〈0.01）. Although there was no association between autoantibody, complemenl and SLEDAI, the LEC in special morphous still associatied with SLEDAI. Conclusion There is a significant association between special morphous LEC and autoantibody, complement as well as SLEDAI and it may be a special parameter that can be used to evaluate the disease activity in SLE patients.