单核细胞趋化蛋白-1在系统性硬化症患者中的表达

Expressions of monocyte chemoattractant protein-1 in systemic sclerosis

目的探讨单核细胞趋化蛋白-1(MCP-1)在系统性硬化症(SSc)的相关性。方法采用酶联免疫吸附试验(ELISA)方法检测27例SSc患者血浆MCP-1水平,并与21名年龄和性别相匹配的健康志愿者血浆中浓度对照,同时采用反转录聚合酶链反应(RT-PCR)和免疫组织化学染色方法检测MCP-1 mRNA和蛋白质在5例SSc患者和3名健康志愿者皮肤成纤维细胞中的表达。结果血浆MCP-1水平SSc组为(787±393)pg/ml,明显高于健康志愿者组的(426±266)pg/ml(P<0.05)。对SSc组的进一步分析显示,11例弥漫型SSc患者血浆MCP-1水平为(896±347)pg/ml,高于16例局限型SSc患者的(714±332)pg/ml (P<0.05):18例并发有肺间质纤维化的SSc患者血浆MCP-1水平(844±327)pg/ml,也高于9例无肺间质纤维化的SSc患者的(676±314)pg/ml(P<0.05)。RT-PCR和免疫组织化学染色的结果显示,5例分离于SSc病变皮肤、在体外培养的成纤维细胞有MCP-1的mRNA和蛋白质分子表达,而3名取自健康志愿者皮肤的成纤维细胞则无表达。结论SSc患者血浆MCP-1水平显著升高,而且与皮肤受累程度和肺间质纤维化有一定的关系。RT-PCR和免疫组织化学染色结果表明,SSc患者病变皮肤组织表达MCP-1增高,提示MCP-1在SSc的病程中起重要作用。

Objective To study the expression of MCP-1 and its correlation with SSc. Methods Twenty-seven patients with SSc and 21 healthy control subjects were examined for MCP-1 expressions by ELISA. mRNA and protein of MCP-1 in fibroblast cells from 5 SSc patients and 3 healthy subjects were also measured by RT-PCR and immunohistochemistry. At the same time, the correlation between the expression levels of MCP-1 and SSc was analyzed. Results The plasma level of MCP-1 was significantly higher in pa- tients with SSc than in healthy control subjects （787±393） pg/ml versus （426±266） pg/ml, P〈0.05. Plasma M CP-1 level in patients with diffuse cutaneous SSc was significantly higher than those in patients with limited cutaneous SSc （896±347） pg/ml versus （714±332） pg/ml, P〈0.05. Plasma MCP-1 level in patients with PF was significantly elevated compared with those in patients without PF （844±327） pg/ml versus （676±314） pg/ml, P〈 0.05. MCP-1 was expressed in fibroblasts from 5 SSc patients and no expression in fibroblasts from 3 normal healthy controls. Conclusion The increase of MCP-1 plasma level from SSc patients, and its correlation with disease activity in SSc indicate that MCP-1 may play an important role in the pathogenesis of SSc.