产超广谱β-内酰胺酶肺炎克雷白杆菌的体外耐药分析

Drug resistance analysis on ESBLs-producing Klebsiella Pneumoniae in vitro

目的探讨儿童肺炎克雷白杆菌产超广谱β-内酰胺酶(ESBLs)的感染情况及耐药特点。方法2004年10月～2006年10月从住院患儿的痰、粪便、尿、血等标本中分离培养出338株肺炎克雷白杆菌,先用纸片扩散初筛法选出产ESBLs可疑菌株后,再用双纸片法确证为产ESBLs菌株。结果ESBLs肺炎克雷白杆菌占全部测试菌的25.4%,ESBLs菌株的耐药率显著高于非ESBLs菌株(P<0.01)。结论早期检测ESBLs菌株,临床可参考药敏选用抗生素治疗此类细菌引起的感染。

Objective To explore the infection and drug resistance of Extended-spectrum β-lactamaseproduing KlebsieUa Pneumoniae （ESBLs-KP） in children, for choosing antibiotic suitably and reasonably. Methods A total of 338 strains of EBSLs-KP were collected during October 2004 to October 2006 from the sputum, feces, urine and blood in pediatric patients of Zhengzhou Children＇ s Hospital. The samples were selected in paper disk diffusion initially, then were determined by the double-paper disk diffusion method. Results The ESBLs-KP was 25.4 % in total, the drug resistance rate of ESBLs was significantly higher than that of non- ESBLs strains（ P 〈 0.01 ）. Conclusion We can select antibiotic for the infection which was caused by the ESBLs strains according to the ESBLs strains early.