血必净注射液对脓毒症大鼠蛋白C及肿瘤坏死因子基因表达的影响

Effects of Xuebijing injection （ 血必净注射液） on protein C and tumor necrosis factor -α mRNA in rats with sepsis

目的探讨血必净注射液对脓毒症大鼠蛋白C（PC）及肿瘤坏死因子-α（TNF—α）基因表达的影响。方法将96只健康Wistar大鼠按随机数字表法分为正常对照组、假手术组、模型组和血必净治疗组，后两组又按处死时间分为术后2、8、24、48和72h亚组，每组8只。采用盲肠结扎穿孔术（CLP）制备脓毒症模型。取腹主动脉血进行血小板计数，并留取肝、肺组织分别检测各组动物组织PC和TNF—α的mRNA表达。结果CLP后8～72h模型组大鼠肝组织PC mRNA表达显著下调（P均〈0．01），血必净注射液可显著提高脓毒症大鼠PC mRNA表达水平（P均〈0．01）。CLP后2h模型组动物肝、肺组织TNF—α mRNA表达均迅速升高并持续至伤后24h（P〈0．05或P〈0．01）；血必净注射液治疗可显著降低肝、肺组织TNF—α mRNA水平（P〈0．05或P〈0．01），术后24h均恢复至伤前正常范围。模型组动物CLP后8～72h血小板计数不同程度减少，血必净治疗组较模型组能明显提高血小板计数（P〈0．05或P〈0．01）。结论血必净注射液可以从基因水平影响脓毒症动物组织PC和TNF—α的mRNA表达。

Objective To investigate the effects of the integrated traditional Chinese medicine Xuebijing injection （血必净注射液） on protein C（PC） and tumor necrosis factor -α （TNF -α） mRNA in rats with sepsis. Methods Sepsis was induced in Wistar rats by cecal ligation and puncture （CLP）. Ninety- six healthy animals were randomly divided into four groups ： normal group, sham - operation group, CLP model group, and Xuebijing- treated group. The two latter groups were divided into 2, 8, 24, 48, and 72- hour subgroups with 8 rats in each subgroup. Platelet count of blood obtained from abdominal aorta was determined and tissue samples from liver and lungs were collected to measure tissue PC and TNF -α mRNA expression. Results PC gene expression levels in lung tissues were significantly lowered （all P〈0. 01）, but they were dramatically raised by Xuebijing injection during 8 -72 hours post -CLP （all P〈0.01）. Compared with normal group, TNF - α mRNA levels in liver and lungs were significantly elevated at 2 hours post - CLP （P〈0.05 or P〈0.01）. However, treatment with Xuebijing injection markedly reduced TNF -α mRNA both in liver and lungs at 2- 24 hours （P〈0.05 or P〈0.01）. In CLP group, blood platelet count was significantly decreased to certain extent at different intervals within 8 - 72 hours, and it was markedly elevated in the Xuebijing - treated group （P〈0. 05 or P〈0. 01）. Conclusion The current study suggests that Xuebijing injection could exert preventing effect on the development of severe sepsis by suppressing PC and TNF -α mRNA.