摘要
目的探讨移植肝细胞在倒千里光碱(RS)处理的大鼠肝脏中的增殖情况。方法将5-溴脱氧尿苷(BrdU)标记的肝细胞通过门静脉途径移植至RS或肝切除处理的大鼠肝脏中,通过免疫组织化学方法检测移植肝细胞数量及流式细胞仪检测S期细胞比率,从而了解供肝细胞在受体内的增殖。结果在接受RS和肝切除处理的大鼠肝脏中,移植的肝细胞数术后3、7、14、28d分别是41±4、67±4、154±10、248±23/1000个细胞,S期细胞比率分别是(4.37±0.21)%、(5.33±0.32)%、(9.55±0.72)%、(17.65±1.76)%,呈上升趋势。结论RS能够为移植肝细胞在受体内的增殖制造足够的增殖空间,在肝损伤造成的肝再生环境中,移植肝细胞的增殖更加显著。
Objective To study the proliferation of transplanted hepatocytes in the liver of retrorsine (RS) -treated rats. Methods BrdU-labeled hepatocytes were transplanted to the RS-treated and/ or partially hepatectomized rats through portal vein. The number of transplanted hepatocytes was counted by using immunohistochemistry and the ratio of S phase cells was detected by using flow cytometry. Results In RS-treated and partially hepatectomized rats ,the transplanted hepatocytes were 41 ±4,67 ±4,154 ± 10 and 248 ± 23/1000 cells, and the ratio of S phase cells was ( 4. 37 ± 0.21 ) % , ( 5.33 ±_ 0. 32 ) %, (9.55 ± 0. 72) % and ( 17.65 ± 1.76) % respectively 3,7,14 and 28 days after operation, showing an ascending trend. Conclusion RS can make enough proliferation room for donor hepatocyte,especially in the liver regeneration environment caused by partially hepatectomy.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第8期929-930,共2页
Chinese Journal of Experimental Surgery
关键词
肝细胞
移植
倒千里光碱
增殖
Hepatocyte
Transplantation
Retrorsine
Proliferation