肺癌中表皮生长因子受体过表达与女性、非吸烟、腺癌和基因突变的关系

Association of epidermal growth factor receptor over-expression with female,non-smoking,adenocarcinoma and gene mutation

目的探讨女性和非吸烟的肺腺癌标本中表皮生长因子受体(EGFR)过表达与EG- FR基因突变的关系。方法应用组织微阵列和免疫组织化学方法检测137例国人非小细胞肺癌标本(鳞癌71例、腺癌66例)中EGFR的表达,采用测序的方法检测EGFR基因突变。结果EG- FR过表达率在肺鳞癌中为63.4%显著高于腺癌的43.9%(P<0.05),其与性别、吸烟情况无关,也与基因突变无关。结论EGFR的过表达率在女性、非吸烟、腺癌及存在EGFR突变的标本中未见升高,提示这些人群中EGFR抑制剂效果较好的原因与蛋白过表达无关。

Objective To evaluate the association of adenoeareinoma, female, non-smoking and epidermal growth factor receptor （EGFR） gene mutations with EGFR expression levels in non-small-cell lung cancer （NSCLC） samples. Methods 137 NSCLC samples （71 squamous cell carcinomas and 66 adenoeareinomas） constructed into tissue micro-array were investigated for EGFR expression by immunohistoehemistry. EGFR mutations in exon 19 and exon 21 were determined by sequencing. Results EGFR was more often over-expressed in squamous cell carcinoma samples than adenoeareinomas （ 63.4% vs 43.9 %, P 〈 0.05 ）. Expression level was not related to gender, smoking status, and EGFR mutational status. Logistic regression also failed to show association of these parameters with EGFR over-expression. Conclusion Higher efficiency of EGFR inhibitors in NSCLC patients of East Asian ethnics, female, non- smoking and tumors carrying EGFR activating mutations can not be attributed to EGFR expression level differences.