白藜芦醇对阿霉素致心衰大鼠心肌损伤的保护作用

Effect of resveratrol on heart function of rats with adriamycin-induced heart failure

目的:观察白藜芦醇(resveratrol,RES)对阿霉素(adriamycin,ADR)致急性心衰大鼠心功能的保护作用。方法:取成年雄性SD大鼠30只,随机分为5组:空白对照组(NC),ADR模型组、RES低剂量(RESL)+ADR组,RES高剂量(RESH)+ADR组及RES组。RESL+ADR,RESH+ADR组及RES组,分别给予RES 30,120,120 mg.kg-1.d-1腹腔注射,1次/d,连续3 d;其他两组腹腔注射等量生理盐水。第4天除NC组及RES组外,其余3组一次性腹腔注射ADR10 mg.kg-1,制备急性心衰大鼠模型。24 h后,光镜观察心肌细胞形态及生化指标的变化。结果:光镜观察显示,RES+ADR组心肌细胞的变性坏死明显减轻。ADR模型组丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(NOS)显著增加,超氧化物岐化酶(SOD)显著减少(P<0.05)。与ADR模型组相比,RESL+ADR,RESH+ADR组的MDA,NO,NOS显著减少,SOD增加(P<0.05),并呈剂量依赖性。结论:RES可显著减轻ADR对心肌的毒性作用,其作用与抗氧化和减少NO的产生有关。

Objective： To observe the protective effects of resveratrol （RES） on the heart function of the rats with adriamycininduced heart failure. Method： Thirty adult male SD rats were randomly divided into 5 groups ： normal control （NC） group, adriamycin （ADR） group, RESL ＋ADR group, RESH ＋ADR group and RES group. RES of 30, 120, 120 mg · kg^-1 · d^-1 was given intraperitoneally （ip） once a day for 3 days in RESL ＋ ADR group, RESa ＋ ADR group and RES group respectively. The other two groups were given the same amount of normal saline the same way. On the 4th day,ADR of 10 mg · kg^-1 was given intraperitoneally once to induce myocardiam injury model. After twenty-four hours, the pathological and biochemical changes of the myocardium were examined. Result ： As compared with NC group, the MDA, NO and NOS of the ADR group were significantly higher （P 〈 0. 05 ）, and the SOD of the ADR group were markedly lower （P 〈0. 05）. As compared with ADR group, the indexes in RESL ＋ ADR group, RESH ＋ ADR group were exactly opposing, and took on dose dependance （P 〈 0. 05 ）. Light microscopic morphometry of the heart samples of the rats in ADR ＋ RESL. a groups revealed typical diminishing of damage. Conclusion： RES can relieve the toxic effects of ADR on myocardiam, and the cardioprotective effects may be correlated with its antioxidant activity and downregnlation of NO.