鹰嘴豆化学成分研究

Studies on chemical constituents in seeds of Cicer arietinum

目的:研究鹰嘴豆的化学成分,从中寻找具有生物活性的天然化合物。方法:采用不同体积分数的乙醇对鹰嘴豆干燥种子及鹰嘴豆发芽种子进行提取,经硅胶、大孔树脂D101、葡聚糖Sephadex LH-20色谱手段分离纯化,通过NMR,MS等波谱分析手段鉴定化合物结构。结果:分离到9个化合物,分别鉴定为3-羟基-齐墩果-12-烯(1),鹰嘴豆芽素A-7-O-β-D-葡萄糖苷(2),脑苷(3),1-乙基-α-L-半乳糖苷(4),尿苷(5),腺苷(6),色氨酸(7),鹰嘴豆芽素A(8),芒柄花素(9)。结论:1,3,4,6,7均为首次从鹰嘴豆属植物中分离到。

Objective： To study the chemical constituents in seeds of Cicer arietinum, so that to find bioactive natural products. Method： Dried and sprouted seeds of C. arietinum were extracted with ethanol of various concentrations respectively, then isolated and purified by silica gel, macroreticular resin D 101, Sephadex LH - 20 gel column chromatography, and structures of compounds were identified by spectral analysis. Result： Nine compounds have been isolated and identified： 3-hydroxy-olean-12-ene （1）, biochanin A-7-O-β-D-glucoside （2）, cerebroside （3）, 1-ethyl-α-L-galactoside （4）, uridine （5）, adenosine （6）, trytophan （7）, biochanin A （8）, fomononetin （9）. Conclusion： Compounds 1, 3, 4, 6, 7 were isolated from genus Cicer for the first time.