桂皮醛对NIH3T3细胞周期及相关蛋白表达的影响

Effects of cinnamyl aldehyde on cell cycle and relafeol proteins expression in NIH3T3 cells

目的:观察肉桂主要成分桂皮醛对小鼠成纤维瘤细胞株NIH3T3细胞周期及相关蛋白的影响。方法:采用流式细胞仪检测桂皮醛(5.5 ng.mL-1)对NIH3T3细胞周期分布的影响;用免疫细胞化学法检测桂皮醛对NIH3T3细胞的细胞周期蛋白D1(Cyclin D1)和增殖细胞核抗原(PCNA)蛋白表达的影响。结果:流式细胞仪检测显示,桂皮醛作用24 h后NIH3T3细胞S期比例上升了3%(P<0.05),G2/M期比例无明显升高,细胞增殖指数(PrI)增加了3.5%(P<0.01)。经桂皮醛刺激后,NIH3T3细胞Cyclin D1和PCNA蛋白表达量明显增加,与对照组比较,有极显著差异(P<0.01)。结论:桂皮醛可推动NIH3T3细胞周期向前进展,这种正向调控作用与桂皮醛能促进Cyclin D1和PCNA蛋白表达有关。

Objective： To observe the effects of Cinnamyl aldehyde （CA） on NIH3T3 cell cycle and explore the possible mechanism further. Method： Flowcytometry was used for observing cell cycle distribution. Expressions of proliferation cell nuclear antigen （PCNA） and Cyclin DI protein in NIH3T3 cells were assessed by immunocytochemistry. Result： After culture with CA for 24 hours, the percentage of populations of S phase was enhanced by 3% （ P 〈 0. 05 ） and cell proliferation index （ PrI, S ＋ G2/M） was increased by 3.5% （P 〈0.01 ）, but G2/M phase had no obvious changes. The expressions of Cyclin D1 and PCNA proteins were im- proved markly by CA compared with controlgroup （P 〈 0. 01 ）. Condusion： CA could promote more cells in G0/G1 phase into S phase, which may be related to the regulation of the expressions of PCNA and Cvclin D1.