双参宁心胶囊对小型猪介入性心肌缺血的保护作用

Protective effects of Shuangshen Ningxin capsule on miniature swine after myocardial ischemia by intervention

目的:研究双参宁心胶囊对小型猪介入性心肌缺血的治疗作用。方法:小型猪LAD经心导管介入自体血栓制备心肌缺血模型,喂饲双参宁心胶囊6 d后,观察冠状动脉造影、血流动力学、生化和病理组织学的改变。结果:造模6 d后,心肌缺血小型猪冠状动脉LAD基本栓塞,心输出量(CO)、心脏指数(CI)、左心做功(LCW)和左心做功指数(LCWI)明显降低,病理组织学可见心肌变性、坏死,缺失区被纤维组织替代填充、嗜中性粒细胞浸润。各组给药6 d后,双参宁心胶囊可明显减轻小型猪冠状动脉LAD自体血栓栓塞的程度,改善血流动力学,增加CO,CI,LCW,LCWI,降低外周血管阻力(SVR)和外周血管阻力指数(SVRI),抗脂质过氧化,增加血浆超氧化物歧化酶(SOD)活性,降低血浆丙二醛(MDA)含量,同时减轻心肌病理组织学变性和坏死改变。结论:双参宁心胶囊可能通过改善心肌收缩功能,增加左心做功,抑制心肌细胞膜的脂质过氧化,发挥抗心肌缺血的作用。

Objective： To study the therapeutical effects of Shuangshen Ningxin capsule on miniature swine after myocardial ischemia by intervention. Method： Myocardial ischemic model miniature swine induced by self-thrombus via cardiac catheter in left anteriar descending coronary artery （ LAD）, were administrated Shuangshen Ningxin capsule for 6 days. The changes of coronary arteriog- raphy, hemodynamics, biochemistry and pathohistology were observed. Result： 6 days after modeling, LAD in myocardial ischemic miniature swine was basically embolized, cardiac output （ CO）, cardiac index （ CI）, left cardiac work （LCW） and left cardiac work index （LCWI） obviously lowed, and pathohistological analysis revealed myocardial degeneration, necrosis, fibrosis and inflammatory cell infiltration. After being administered with shuangshen Ningxin capsule 6 days, the degree of self-thrombus blocked LAD reduced, hemodynamic indexes of CO, CI, LCW, LCWI and blood plasm superoxide dismutase （SOD） activity increased, and systemic vascular resistance （ SVR）, systemic vascular resistance index （SVRI） and malondialdehyde （MDA） content were lowed, on the same time, pathohistological di^generation and necrosis reduced. Conclusion： Shuangshen Ningxin capsule has anti-myocardial ischemia effect by improving cardiac muscle systolic function, increasing left cardiac work, inhibiting cardiac muscle cellular membrane lipid peroxidation.