摘要
目的:研究钙对铅染毒大鼠骨钙素(OC)、甲状旁腺激素(PTH)及其受体(PTHr1)表达的影响。方法:分别以纯净水和1.0g/L醋酸铅水饲喂Wistar大鼠,其中醋酸铅饲喂大鼠又分为正常饲料、高钙饲料和低钙饲料组。测定大鼠骨钙、骨铅、OC和PTH含量,并采用RT-PCR方法观察OC和PTHr1mRNA的表达。结果:铅处理降低了骨钙和血清OC的含量,抑制了OC mRNA的表达;但同时血清PTH的含量升高,PTHr1的表达增强;而高钙可以拮抗铅的这些作用,低钙则加剧了铅的上述效应。结论:补充膳食钙可减轻铅对大鼠骨骼的毒性作用。
Objective： To study the effect of calcium on osteocalcin（OC） and parathyroid hormone （PTH） in rat exposed to lead. Method： Wistar rats were randomly divided into control group, lead group, low-calcium＋lead group and high-calcium＋lead group. The content of calcium and lead in bone, OC and PTH of rat with different treatments were detected. At the same time, RT-PCR analysis was used to determine the expression of OC and PTHr1. Results： Lead decreased the content of calcium in bone and OC. The expression of OC was inhibited, and the level of PTH and the expression of PTHr1 increased, which could be enhanced by low calcium treatment, but high calcium treatment could antagonize these effects of lead. Conclusion： Supplementation of calcium in diet could extenuate the toxicity of lead on bone in rat.
出处
《营养学报》
CAS
CSCD
北大核心
2007年第4期328-331,共4页
Acta Nutrimenta Sinica
基金
中国营养学会营养科研基金(2003)
关键词
钙
铅
骨钙素
甲状旁腺激素及其受体
calcium
lead
osteocalcin
parathormone
parathormone receptor
