牙源性钙化囊肿的血管内皮细胞Ⅷ因子相关抗原及PCNA的免疫组织化学研究

An immunohistochemical study of calcifying odontogenic cyst by using factor Ⅷ antigen marking vessel endothelial cell and PCNA

为了解牙源钙化囊肿的囊肿型和肿瘤型内血管和细胞增殖活性之间的关系，本研究应用血管内皮细胞的标志性抗原VIII因子及增殖细胞核抗原（PCNA），对11例牙源性钙化囊肿进行了免疫组织化学研究，并以6例造釉细胞癌作对照。结果显示：囊肿型牙源性钙化囊肿的部分标本中，囊壁上皮下有VIII因子染色阳性的密集的新生小血管，囊壁上皮中PCNA染色阳性细胞也较多，显示囊壁上皮增殖活动较强的组织依，部分囊壁上皮下血管较少，PCNA染色阳性细胞也较少，显示囊壁上皮增殖较慢，相对稳定的组织学像。而在肿瘤型牙源性钙化囊肿中，肿瘤间质中VIII因子杂色阳性血管密度明显增加，并且血管管腔增大，PCNA染色见肿瘤的上皮团片中阳性细胞也明显增多，反映了肿瘤增殖活跃，生长较快。表明肿瘤内血管的增生与肿瘤的增殖有密切关系。

The formation of the vessel in the neoplastic mesenchymal was the first condition for the lasting growth of tumors, and it was of supporting and nurturing. In this study 11 cases calcifving odontogenic cysts (COC) and 6 cases ameloblastomas were investigated by using immunohistochemical method to detect factor VIII antigen marking vessel endothelial cell and proliferating cell nuclear antigen (PCNA). The results showed some cells were positive reaction to PCNA and the number of small vessels underlying cystic walls increased in the cyst type of COC. This suggested that the cysts proliferated actively and formed solid epithelial masses gradually, finally developed tumors. Some cysts proliferated slowly and maintained cyst nature. In the tumor type of COC, PCNA-positive cells and mesenchymal vessels were obviously rich. That indicated the tumor proliferated actively and grew rapidly. In the mean time, tumor development was studied by investigating the proliferation degree between neoplastic cells and mesenchymal vessels.