摘要
以金膜为免疫检测的基底,采用自组装技术(Self-assembled monolayer,SAM)将ω-巯基十六酸(16-MHA)修饰于金膜后与抗体结合成固相抗体,在此基础上组装“固相抗体-待测抗原-标记免疫金溶胶”三明治复合体系.采用不同标记分子苯硫酚(Thiophenol)和4,4′-联吡啶(4,4′-Bipyridine)分别标记不同的免疫金溶胶,利用表面增强拉曼光谱(SERS)谱峰较窄且具有较强的分辨率及高灵敏度的特点,通过对两种标记分子特征谱峰的判断识别所加入的两种抗原.通过选择合适的标记分子和一定尺度的免疫溶胶,标记免疫SERS检测的检测限可达到飞克级(1~100 fg/mL).
Au film which was used as immunoassay substrate was modified by 16-mercaptohexadecanoic acid (16-MHA) via self-assembled monolayer(SAM) technology. A sandwich assay which contains captured antibodies, antigens and labeled immuno-colloids was formed. Two different Raman reporter molecules (4,4'-bipyridine and thiophenol), which served as respective labels for each type of antibodies, were selected. SERS has the advantage of a small line width of Raman bands and high sensibility. The presence of dual-analyte was established by distinguishing the corresponding labeled reporter's Raman spectrum. By chosing suitable Raman reporter molecules and immuno-colloids of certain size, detection limit ranges from 1 to 100 fg/mL were achieved.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2007年第8期1464-1468,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20373046
20573076)
厦门大学固体表面物理化学国家重点实验室开放课题资助
关键词
表面增强拉曼光谱
标记免疫检测
双组分
检测限
Surface enhanced Raman spectroscopy (SERS)
Labeled immunoassay
Dual-analyte
Detection limit