藤三七中一个新黄烷醇和抗HIV活性成分

One New Flavanoid and Anti-HIV Active Constituents from Boussingaultia gracilis Miers var. pseudobaselloides Bailey

利用各种色谱（硅胶和凝胶）方法,从藤三七[Boussingaultia gracilisMiers var.pseudobaselloidesBailey]的70%（体积分数）的乙醇提取物中分离得到2个黄烷醇类化合物（1,2）和4个黄酮类化合物（3～6）.采用UV,IR,MS和1D,2D NMR方法,分别鉴定出如下化合物：7-羟基-5-甲氧基-8-甲基-6-甲酰基-3,4-黄烷二醇,命名为藤三七醇A（1）;4,7-二羟基-5-甲氧基-8-甲基-6-甲酰基黄烷（2）;7-O-methylunonal（3）;5,7-二羟基-6,8-二甲基-2-苯基-4H-1-苯并吡喃-4-酮（4）;Desmosflavone（5）和Demethoxymatteucinol（6）.其中化合物1是一个新的黄烷二醇化合物,化合物2～6为首次从该植物中分离得到.抗HIV-1活性筛选结果表明：化合物1,2,5,6对HIV-1诱导合胞体的形成具有一定的抑制作用,其半数有效浓度（EC50）分别为45.09,48.73,55.47和82.75μmol/L,治疗指数（TI）分别为1.41,1.20,7.15和〉8.51.

Boussingauhia gracilis Miers var. pseudobaselloides Bailey（Basellaceae） is a folk medicine used as an analgesic and supplements, only a few research was reported on the chemical constituents of this plant. This paper presented its chemical and anti-HIV active constituents. By column chromatography （Silica and Sephadex LH-20 gel） methods, two flavanols and four flavones were isolated from the 70% （volume fraction） ethanol extract. Based on extensive spectral analysis（UV, IR, MS and 1D, 2D NMR）, compounds 1-6 were identified as bougracol A （ 1 ）, 4,7-dihydroxy-5-methoxy-8-methyl-6-formyl-flavane （ 2 ）, 7-O-methyl- unonal（3）, 5,7-dihydroxy-6,8-dimethyl-2-phenyl-4H-1-benzopyran-4-one（4）, desmosflavone（5） and demethoxymatteucinol（6）. Among them, compound 1, a new flavan-diols, was deduced to be 7-hydroxy-5-methoxy-8-methyl-6-formyl-flavan-3,4-diols, and compounds 2-6 were firstly obtained from this plant. Anti- HIV assay suggests that compounds 1, 2, 5, 6 showed weak anti-HIV activities with EC50 values of 45.09, 48. 73, 55.47 and 82. 75μmol/L, and TI values of 1.41, 1.20, 7. 15 and 〉8.51, respectively.