摘要
利用各种色谱(硅胶和凝胶)方法,从藤三七[Boussingaultia gracilisMiers var.pseudobaselloidesBailey]的70%(体积分数)的乙醇提取物中分离得到2个黄烷醇类化合物(1,2)和4个黄酮类化合物(3~6).采用UV,IR,MS和1D,2D NMR方法,分别鉴定出如下化合物:7-羟基-5-甲氧基-8-甲基-6-甲酰基-3,4-黄烷二醇,命名为藤三七醇A(1);4,7-二羟基-5-甲氧基-8-甲基-6-甲酰基黄烷(2);7-O-methylunonal(3);5,7-二羟基-6,8-二甲基-2-苯基-4H-1-苯并吡喃-4-酮(4);Desmosflavone(5)和Demethoxymatteucinol(6).其中化合物1是一个新的黄烷二醇化合物,化合物2~6为首次从该植物中分离得到.抗HIV-1活性筛选结果表明:化合物1,2,5,6对HIV-1诱导合胞体的形成具有一定的抑制作用,其半数有效浓度(EC50)分别为45.09,48.73,55.47和82.75μmol/L,治疗指数(TI)分别为1.41,1.20,7.15和〉8.51.
Boussingauhia gracilis Miers var. pseudobaselloides Bailey(Basellaceae) is a folk medicine used as an analgesic and supplements, only a few research was reported on the chemical constituents of this plant. This paper presented its chemical and anti-HIV active constituents. By column chromatography (Silica and Sephadex LH-20 gel) methods, two flavanols and four flavones were isolated from the 70% (volume fraction) ethanol extract. Based on extensive spectral analysis(UV, IR, MS and 1D, 2D NMR), compounds 1-6 were identified as bougracol A ( 1 ), 4,7-dihydroxy-5-methoxy-8-methyl-6-formyl-flavane ( 2 ), 7-O-methyl- unonal(3), 5,7-dihydroxy-6,8-dimethyl-2-phenyl-4H-1-benzopyran-4-one(4), desmosflavone(5) and demethoxymatteucinol(6). Among them, compound 1, a new flavan-diols, was deduced to be 7-hydroxy-5-methoxy-8-methyl-6-formyl-flavan-3,4-diols, and compounds 2-6 were firstly obtained from this plant. Anti- HIV assay suggests that compounds 1, 2, 5, 6 showed weak anti-HIV activities with EC50 values of 45.09, 48. 73, 55.47 and 82. 75μmol/L, and TI values of 1.41, 1.20, 7. 15 and 〉8.51, respectively.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2007年第8期1508-1511,共4页
Chemical Journal of Chinese Universities
基金
云南省2005~2007人才培养项目
云南省科技厅新药专项(批准号:2004XY01)
国家'十五'科技攻关计划(批准号:2004BA719A14)资助
关键词
藤三七
藤三七醇A
黄烷醇
抗HIV活性
Boussingauhia gracilis Miers var. pseudobaselloides Bailey
Boussingol A
Flavanoid
Anti-HIV activity