Cecropin B抗菌肽接枝丝素蛋白膜的制备和表征

Preparation and Characterization on Cecropin B Peptide Grafted with Silk Fibroin Films

以体积分数为60%的乙醇处理制得不溶性再生丝素膜,通过碳二亚胺法将Cecropin B抗菌肽共价接枝到丝素膜表面,利用红外光谱、扫描电镜、X射线能谱和抗菌性测试等手段对制得的丝素膜结构和表面特性及其抗菌性能进行了测试分析.结果表明,Cecropin B抗菌肽成功地接枝到了丝素膜的表面,接枝后的丝素膜水溶性低,并具有良好、持久的抗菌能力.

The undisolvable Bombyx mori silk fibroin film was prepared from the regenerated silk fibroin solution followed with the 60% （volume fraction） ethanol aqueous solution treatment. The silk fibroin film was grafted with Cecropin B peptide via the carbodiimide chemistry. The secondary structure, the film surface properties and the bacteriostatic activity of the obtained silk fibroin film were analyzed with FTIR, SEM, EDS and the antibacterial tests. The results show that the Cecropin B peptide is covalently grafted onto the surface of silk fibroin film, which is undisolvable and has an effective and permanent bactefiostatic activity.