慢性胃炎、异型增生、胃癌幽门螺杆菌感染与iNOS表达关系的研究

Study on the Relationship between HP Infection and Expression of iNOS in Gastric Carcinoma,Gastric Epithelial Dysplasia and Inflammatory Gastric Musosa

目的：探讨慢性胃炎、异型增生、胃癌幽门螺杆菌（HP）感染与诱导型NO合酶（iNOS）表达的关系。方法：选取我院2002～2005年胃粘膜组织标本124例,同时采集血清。其中慢性胃炎（Ⅰ组）58例,异型增生（Ⅱ组）41例,胃癌（Ⅲ组）25例。各组均采用酶联免疫法及免疫组化法检测HP抗体和iNOS表达水平。结果：iNOS的表达随胃粘膜病变的进展逐级增高,且HP阳性者iNOS表达高于HP阴性者（P〈0.05）。结论：iNOS可能参与了HP感染引起的胃粘膜癌变过程并发挥了重要作用。

Objective：To investigate the expression of iNos in different gastric mucosa lesion and its relationship with the infection of HP. Methods：The total gastric tissues and the corresponding blood serum had been collected from 124 cases, including 58 cases with inflammatory gastric mucosa（group Ⅰ ）,41 cases with gastric epithelial dysplasia（group Ⅱ ） and 25 cases with gastric carcinoma（group Ⅲ ）. In all eases,Immunohistochemical streptavidin/peroxidase （SP） method and ELISA were used to detect the expression of iNos and the HP antibody in blood serum respectively. Results：The expression of iNos increased along with the progression of gastric neoplasia. In HP positive group, the expression of iNos was higher than that in HP negative group （P〈0. 05）. Conclusion： The expression of iNos might participate in the process of gastric carcinogenesis coursed by HP infection and play an important role.