骨组织工程快速成型支架改性的相关研究

Surface modification of rapid prototyping scaffolds for bone tissue engineering

目的探讨胶原杂化及磷灰石表面沉积改性后的聚乳酸一羟基乙酸共聚物／β-磷酸三钙（PLGA／β—TCP）作为快速成型支架应用于骨组织工程的可行性。方法使用骨髓基质干细胞对胶原杂化及磷灰石表面沉积改性后的PLGA／β-TCP的生物相容性进行评估，通过扫描电镜观察改性后材料的表面特性及细胞与材料复合的形态学特征；细胞与材料复合后的繁殖与分化能力分别使用细胞计数及碱性磷酸酶定量方法进行评估。结果通过对亲水性、细胞增殖能力及碱性磷酸酶测定证实改性后的PLGA／β-TCP快速成型支架较单纯材料其亲水性及生物相容性有明显提高（P〈0．05）。结论胶原杂化及磷灰石表面沉积改性后的PLGA／β—TCP快速成型支架可作为三维支架应用于骨组织工程。

Objective To investigate the feasibility of using a novel scaffold composed of poly （lactic-co-glycolic acid） （PLGA）/ β-tricalciumphosphate （β-TCP） skeleton the surface of which is modified with apatite/collagen sponge. Methods BMSCs were used to evaluate the behavior of the cells on the biomimetic coating. The morphology of cells on the surface of PLGA/β-TCP scaffold and on the PLGA/β-TCP scaffold with apatite/collagen sponge composite coating was studied by scanning electron microscopy （SEM）. Cell viability was assessed by cell number counting. The differentiated osteoblastic function was assessed by measuring alkaline phosphatase activity of the cells. Results The apatite/collagen sponge composite coating could improve cell proliferation and ALP activity of the hybrid scaffolds and increase its hydrophilicity after hybridization. Conclusion The PLGA/β-TCP scaffold with apatite/collagen sponge composite coating is promising as a candidate 3D substrate in bone tissue engineering.