骨髓基质干细胞在IKVAV多肽纳米纤维凝胶表面增殖、黏附及向神经细胞诱导分化的研究

The proliferation, adhesion and induced differentiation into neurocytes of bone marrow stromal cells on IKVAV peptide nanofiber gel

目的研究骨髓基质干细胞（BMSCs）与IKVAV多肽纳米纤维凝胶复合的细胞假体应用于脊髓损伤治疗的可行性。方法合成IKVAV多肽两亲性分子，进行自组装，用透射电镜检测。将IKVAV多肽纳米纤维凝胶与BMSCs复合培养，采用倒置显微镜下观察、Calcein—AM／PI染色、CCK-8法、免疫荧光双标法，检测IKVAV多肽对BMSCs增殖、黏附及向神经细胞方向诱导分化的影响。结果IKVAV多肽可成功自组装成纳米纤维凝胶，其与BMSCs复合培养细胞生长良好，活细胞数达90％以上，IKVAV多肽对BMSCs增殖没有影响，可促进BMSCs的黏附，并在诱导BMSCs向神经细胞分化过程中提高神经元分化比例。结论BMSCs在IKVAV多肽纳米纤维凝胶材料表面可良好的增殖及黏附，并可提高其向神经细胞分化过程中神经元的分化比例。

Objective To study the proliferation, adhesion and induced differentiation into neurocytes of bone marrow stromal cells （BMSCs） on IKVAV （isoleucine-lysine-valine-alanine-valine） peptide nanofiber gel in hope that the mixture composed of BMSCs and IKVAV peptide nanofiber gel can be potentially used in spinal cord injury. Methods IKVAV-PA was synthesized and self-assembled to form nanofiber gel which was tested with transmission electron microscope. BMSCs were cultured with IKVAV peptide. The proliferation, adhesion and induced differentiation of BMSCs were observed with inverted microscope, Calcein-AM /PI staining, Cell Counting Kit-8 and immunofiuorescence staining. Results IKVAV peptide self-assembled to form nanofiber gel successfully. BMSCs cultured with IKVAV peptide grew well and the viable count was above 90%. IKVAV peptide did no hinder the proliferation of BMSCs but promoted the adhesion of BMSCs and raised the rate of neurons during the induced differentiation of BMSCs into neurocytes. Conclusion IKVAV peptide nanofiber gel can promote the proliferation and adhesion of BMSCs, and pro- duce an increased rate of neurons during the induced differentiation of BMSCs into neurocytes.