摘要
目的 用DOI诱导大鼠头部抽动建立Tourette综合征动物模型,观察该模型大鼠脑内前额叶和纹状体多巴胺D1、D2受体基因表达。方法 将20只Wistar大鼠随机分为实验组和对照组,实验组大鼠腹腔注射DOI,对照组注射相同体积的生理盐水,连续给药21d。RT-PCR法检测大鼠纹状体、前额叶皮层的多巴胺D1、D2受体基因表达。结果 实验组前额叶D1受体mRNA表达的相对灰度值（0.897±0.378）与对照组（0.417±0.299）相比增高,有显著统计学差异（P〈0.05）,纹状体D1受体mRNA表达无明显变化[（1.162±0.159）vs（0.996±0.479）],实验组前额叶和纹状体D2受体mRNA表达均无明显变化。结论 DOI诱导的Tourette综合征动物模型大鼠前额叶皮层多巴胺D1受体基因表达增高。
Objective To develop animal model of Tourette syndrome （TS） in rats with 1-（ 2,5-dimethoxy-4-iodophenyl）-2-aminopropane （DOI） induced head-shakes ,and study the dopamine D1, D2 receptors mR- NA expression in the animal model. Methods 20 Wistar rats were divided into experimental group and control group randomly. The rats administrated DOI by peritoneal injection used as experimental group, and the rats injected with same volume normal saline used as the control group. After 21 days of intraperitoneal treatment, the dopamine D1 , D2 receptors mRNA expression in striatum and prefrontal lobe cortex of the rats were measured by RTPCR. Results DOI significantly increased dopamine D1 receptor gene expression in prefrontal lobe （0. 897 ± 0.378 ） as compared to saline-treated control group（0. 417 ± 0.299）. In contrast, DOI did not significantly influence dopamine D1 （1. 162 ±0. 159 vs 0.996 ±0.479） and dopamine D2 receptors mRNA content in striatum and dopamine D2 receptors in prefrontal lobe. Conclusion Prefrontal lobe cortex dopamine D1 receptors mRNA expression increased in rats of DOI-induced head-shakes.
出处
《中国行为医学科学》
CSCD
2007年第3期196-198,共3页
Chinese Journal of Behavioral Medical Science
基金
国家自然科学基金资助项目（30471842）
