头穴透刺对急性脑梗死大鼠神经丝蛋白-200的影响

Influence of Scale Point-through-point Acupuncture on 200 kDa Neurofilament Protein in Rats with Acute Cerebral Infarction

目的研究头穴透刺对急性脑梗死大鼠神经丝蛋白-200(NF-200)的影响,探讨针刺对脑梗死大鼠神经可塑性影响的机制。方法将健康雄性Wistar大鼠随机分为假手术组(A组)、模型组(B组)、针刺组(C组)。通过建立大鼠局灶性脑缺血模型(MCAO),用逆转录-聚合酶链反应(RT-PCR)法,测定以上各组在7 d、14 d、28 d不同时间点NF200 mRNA变化情况。结果头穴透刺组脑组织NF-200的表达与假手术组、造模组相比差异有统计学意义(P<0.05);而在不同时间窗内头穴透刺组,模型组与假手术组比较差异有统计学意义(P<0.01)。表明头穴透刺可以促进脑组织神经丝蛋白-200的表达。结论头穴透刺能够提高脑缺血后神经功能,促进肢体功能恢复,增加神经丝蛋白-200的表达,发挥对脑组织神经细胞可塑性的调节作用。

Objective To investigate the influence of scale point-through-point acupuncture on 200 kDa neurofilament protein in rats with acute cerebral infarction and explore the mechanism of the influence of acupuncture on neuronal plasticity in cerebral infarction rats. Methods Healthy male Wister rats were randomly allocated to sham operation （A）, model （B） and acupuncture （C） groups. A rat model of focal cerebral ischemia （MCAO） was made. NF-200 mRNA was measured by reverse transcriptase polymerase chain reaction （RT-PCR） in each group at 7,14 and 28 days. Results The cerebral expression of 200 kDa neurofilament protein in group C was significantly different from that in group A and B （ P 〈 0.05 ） ; there was a significant difference between group C and B or A at different time windows （P 〈 0.01 ）, indicating that scale point-through-point acupuncture could improve the cerebral expression of 200 kDa neurofilament protein. Conclusion Scale point-through-point acupuncture can improve neural function, promote the recovery of and limb function and increase the excpression of 200 kDa neurofilament protein after cerebral ischemia, producing a regulative effect on neuronal plasticity in the brain.