生长抑素对急性坏死性胰腺炎大鼠胆碱能抗炎通路的影响

The effect of somatostatin on the cholinergic anti-inflammatory pathway of acute necrotizing pancreatitis in rats.

目的探讨生长抑素（SS）对ANP大鼠血清乙酰胆碱酯酶（true choline esterase,TChE）和乙酰胆碱转移酶（choline acetyltransferase,ChAT）及IL-6和TNF-α水平的影响。方法将30只SD大鼠随机分成对照组、ANP组和SS组,每组10只。以胆胰管注入牛胆酸制备ANP模型,SS组于制模后即刻皮下注射SS34μg/kg体重。检测各组血WBC、血糖及血清ALT、AST、LDH、淀粉酶、TNF-α、IL-6、TChE、ChAT含量。结果ANP组血WBC、ALT、AST、LDH、淀粉酶、IL-6、TNF-α及TChE水平较对照组明显升高,而血清ChAT则明显降低（P〈0.01）;血清IL-6、TNF-α与ChAT呈负相关（r=-0.87,P=0.007;r=-0.96,P=0.000）,与TChE呈正相关（r=0.98,P=0.000;r=0.89,P=0.004）。SS组的血WBC、ALT、AST、LDH、淀粉酶、IL-6、TNF-α及TChE水平较ANP组显著降低,而血清ChAT则显著升高（P〈0.01）。3组血糖含量无显著差异。对照组、SS治疗组和ANP组血WBC、ALT、AST及LDH依次升高（P〈0.01）。结论SS可能通过增加ANP大鼠ChAT活性和降低TChE活性,减少IL-6、TNF-α分泌,从而减轻SIRS和脏器功能受损的程度。

Objective To investigate the effect of somatostatin （SS） on the serum level of true choline esterase（TChE）and choline aeetyltransferase（ChAT）, interleukin-6 （IL-6） and Tumor Necrosis Factor-a（TNF-α） in rats with acute necrotizing pancreatitis （ANP）. Methods 30 SD rats were randomized to 3 groups （control group, ANP group and SS-treated group）. Each group included 10 rats. ANP model was induced by retrograde injection of sodium taurocholate into the biliary and pancreatic duct. SS was injected subcutaneously soon after the induction. The blood routine, biochemistry, amylase, liver enzyme, TNF-α, IL-6, TChE and ChAT were observed. Results The serum IL-6, TNF-α and TChE were significantly higher in ANP group than those in control group （P 〈 0.01）. The serum ChAT was significantly lower in ANP group than that in control group（P 〈 0. 01）. The correlation analysis indicated that the IL-6 and TNF-α were positively correlated with TChE （r=0.87, P = 0. 007; r = 0.96,P = 0. 000）and negatively correlated with ChAT （r = 0.98,P = 0. 000; r = 0.80, P = 0. 004）. The serum white blood cell, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase, IL-6, amylase, TNF-α and TChE were significantly lower in SS-treated group than those in ANP group（P 〈 0.01）. The serum ChAT was significantly higher in SS-treated group than that in ANP group（P 〈 0. 01）. The serum levels of white blood cell, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase in SS-treated group were significantly lower than those in ANP group and significantly higher than those in control group （P 〈 0. 01）. Conclusions SS may decrease the secretion of IL-6 and TNF-αby increasing ChAT activity and decreasing TChE activity, thus may re lieve systemic inflammatory response syndrome and reduce the degree of damaged function of organs.