抑癌基因PTEN在胃癌中的表达及临床意义

Expression and clinical significance of anti-oncogene PTEN in gastric carcinoma

目的探讨胃癌组织中PTEN基因表达与胃癌临床病理的关系及其对预后的判断价值。方法应用免疫组化SP技术和图像分析技术,研究PTEN在胃癌和正常胃黏膜组织中的表达情况及阳性表达面积、强阳性表达面积,分析与病理参数的关系,并采用Kaplan-Meier生存曲线分析不同表达水平对术后生存率的影响。结果发现胃癌组织PTEN表达阳性率、阳性面积、强阳性面积均明显低于正常胃黏膜组织(P<0.01)。胃癌组织PTEN表达与胃癌组织分化程度、浸润深度、淋巴转移、肿瘤分期明显相关,术后3、5 a生存率低表达者明显低于高表达者(P均<0.05)。结论PTEN基因表达低下或丢失与胃癌发生、浸润、转移有关,可作为预测术后生存率的指标。

[ Objective] To study expression of anti-oncogene phosphatase and tensin homolog deleted on chromosome ten （PTEN） and its clinical significance in gastric carcinoma pathology and prognosis. [ Methods] SP immunohistochemistry and image analysis were used for studying the gene expression, positive expression area and strong positive expression area of PTEN in gastric carcinoma tissue and normal gastric mucosa tissue. The influence of postoperative survival rates in different expression level was analyzed with Kaplan-Meier survival curves. [ Results] Positive percentage, positive expression area and strong positive expression area of PTEN expression in gastric carcinoma tissue were much higher than those in normal gastric mucosa tissue （ P 〈0. 01 ）. Expression of PTEN in gastric carcinoma tissue was related to differentiation degree, infiltration depth, lymphatic metastasis and tumor stage in gastric carcinoma tissue obviously. As for postoperative survival rates for 3 and 5 years, it was obviously that the hypo-expressers were lower than hyper-expressers. [ Conclusion] Decreased or lost expression of anti-oncogene PTEN correlates with occurrence, infiltration and metastasis in gastric carcinoma, and it can be used as a prognosis indication for of postoperative survival rates.