米团花色素的毒理学研究

Toxicological research on yellow pigment prepared from Leucosceptrum canum(Smith)

采用热水提取，获得色价为1040U／mL的米团花色素，利用小鼠进行急性毒性（LD50）、微核试验、精子畸变和Ames试验等毒理学试验项目。结果表明：雌雄小鼠LD50〉21．5g/kgBW，属无毒级物质；2.50～10．0g／kg BW剂量的微核试验和精子畸变试验结果为阴性，受试物对体细胞和生殖细胞无诱变作用；Ames试验结果为阴性，受试物无直接或间接的致突变作用。

Yellow pigment with color value 1 040 U/mL was extracted by water at 85 ℃ from flower of Leucosceptrum canum （Smith） and toxicological research was carried out in the Center of Disease Control of Guangdong Province. NIH mouse（ SPE grade） was given orally at dose of 2.15, 4.64,10.0,21. 5 g/kg BW and it was found that the LD50 value of the pigment was more than 21. 5 g/kg BW, classified as non-toxic grade. No mutation effect for body cell and reproductive cell was observed at dose of 2.5 to 10.0 g/kg BW respectively by bone marrow microleues assay and sperm shape abnormality test. Ames test was negative.