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人类免疫缺陷病毒确认试验不确定结果分析 被引量:30

An analysis on indeterminate results of human immunodeficiency virus in western blot
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摘要 [目的]通过对104例人类免疫缺陷病毒(HIV)抗体免疫印迹法试验(WB)不确定结果的分析,探讨HIV抗体不确定结果的处理方法,明确个体诊断。[方法]对104例样本采取回顾性调查和部分病例前瞻性随访观察相结合的方法,综合分析样本来源、有无高危行为、酶联免疫吸附试验的吸光度值与临界值的比值、条带带型特点与最终转归的相互关系。[结果]104例不确定样品主要来源于无偿献血和单采浆样品,分别占46.15%(48/104)和31.73%(33/104);吸光度值与临界值的比值(S/CO值)主要集中于1-4之间,占总数的83.65%(87/104),S/CO值大于6的比例为5.77%(6/104);不确定样品的带型分布特点以p24居多,占总数的70.19%(73/104)。2例转为确定者的S/CO值均小于6,带型分别为gp160和p24。[结论]在低流行地区的低感染率人群中,不确定结果显示其感染HIV的可能性不大;对具有明确高危行为者,虽然其检测结果S/CO值小于6,带型反应也不是很强,但仍有感染的可能性,需加强随访,并可开展相应的辅助诊断。 [ Objective ] To understand the indeterminate results of Western - Blot of Human immunodeficiency virus ( HIV) antibody as to conclude the diagnosis. [ Methods] Comprehensive analysis of 104 cases were used to determine the relationship between source of sample, risk behavior, proportion of optical density value and cut - off value [ S/CO) of ELISA, bands of Western - Blot and final sero - conversion. [ Results] Among 104 cases, 46.15% and 31.73% were from blood donors and plasma donors respectively. Most of S/CO values of ELISA were 1 -4 occupied 83.65%. 6 cases had S/CO value more than 6. The main bands of Western -Blot was p24, occupied 70.19%. 2 cases were determined as sero -positive with S/CO value more than 6 and weak bands of gpl20 and p24. [ Conclusion] Most of the indeterminate results of Western - Blot samples were sero -negative. The medical follow- up and complementary diagnosis should be given to those with risk behavior and weak typical bands of Western - Blot.
出处 《上海预防医学》 CAS 2007年第8期373-376,共4页 Shanghai Journal of Preventive Medicine
关键词 人类免疫缺陷病毒 高危行为 随访 Human immunodeficiency virus High rish behavior Follow up
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