非霍奇金淋巴瘤中PTEN和PCNA表达的临床、病理关系研究

Investigation of the clinical and pathological relationship bitween PTEN and PCNA in Non-Hodgkin's Lymphoma

目的探讨PTEN和PCNA在非霍奇金淋巴瘤(non-hodgkin’slymphom,NHL)中的表达及临床意义。方法采用S-P免疫组织化学方法检测了在50例NHL和12例淋巴结反应性增生(reactive lymphoid hyperplasia,RH)标本中PTEN和PCNA的表达情况。结果(1)PTEN在NHL中的表达率均明显低于在RH中的表达率,且随着NHL恶性程度增加,PTEN表达率明显下降(P<0.05);(2)与RH相比,NHL中PCNA明显呈过表达(P<0.05),而且PCNA阳性强度随着NHL恶性程度的增加而增高;(3)PTEN与PCNA表达呈负相关(P<0.05),而与患者性别、年龄、免疫分型、临床分期及肿瘤原发部位等因素无明显相关(P>0.05)。结论PTEN和PCNA都与NHL的发生、发展有关,联合检测PTEN和PCNA水平在对NHL的诊断、治疗及预后判断中具有重要的临床意义。

Objective To investigate the expression of FFEN and PCNA in non Hodgkin＇ s lymphoma （NHL） and its significance. Melhods The expression of FTEN protein and PCNA antigen were measured with technique in 50 cases of NHL and 12 cases of reactive lymphoid hyperplasia（RH）. Results （1）The expression of FFEN is much more lower in NHL than that in RH,and the expression of FFEN de- creasing was accompanied with grade increasing of NHL（ P 〈 0.05）. （2）Comparing with RH, tumor tissue as- sume overexpression of PCNA significantly （ P 〈 0.05）. The positive degree of PCNA increaseed witl!, the malig- nant degree increasing of NHL. （3）FFEN is inversely related to PCNA expression （ P 〈 0.05） .The expressions of FFEN and PCNA didn＇t show correlation with such factors as sex, age, immunophenotype, clinical phase and location in NHL （ P 〉 0.05）. Conclusion FFEN and PCNA are closely related to the development and pro- gression of NHL. To detect FFEN and PCNA levels unitedly can provide useftd clinical value for diagnosis, thera- py and prognostic predicting in NHL.