2型糖尿病微血管病变患者血浆对氧磷酯酶-1活性与氧化应激的关系

The Relationship Between Plasma Paraoxonase-1 and Oxidative Strees in Type 2 Diabetes with Microangiopathy

目的探讨2型糖尿病微血管病变(DMPA)患者血浆对氧磷酯酶-1(PON-1)活性与氧化应激的关系。方法168例2型糖尿病根据微血管并发症情况分为无微血管并发症(NDC)组、糖尿病视网膜病变(DR)组和糖尿病肾病(DN)组,分别检测各组患者血浆PON-1、超氧化物歧化酶(SOD)和循环谷胱甘肽过氧化物酶(GSHPx)活性、丙二醛(MDA)含量,分析PON-1活性与SOD、MDA、GSHPx之间的关系。结果糖尿病各组患者血浆PON-1、SOD、GSHPx活性均明显低于对照组(P<0.01),DMPA组又低于NDC组(P<0.05),而血浆MDA含量与对照组比较显著升高(P<0.01),DMPA组又高于NDC组(P<0.05)。相关分析表明,糖尿病患者血浆PON-1活性与GSHPx、SOD呈正相关(r=0.774,P<0.01;r=0.801,P<0.01),与MDA呈负相关(r=-0.833,P<0.01)。结论在2型糖尿病DMPA中氧化应激增强及血浆PON-1活性显著降低,PON-1与氧化应激及其相互作用参与了2型糖尿病DM-PA的发生和发展。

Objective To investigate the relationship between the avtivity of plasma paraoxonase-1 （PON-1） and oxidative stress in type 2 diabetes with microangiopathy. Methods 168 cases of type 2 diabetes mellitus were divided into three groups according to vessel lesions： no-diabetic complication group（NDC）, diabetic retinopathy group（DR） and diabetic nephropathy group（DN）, and matched with normal controls（NC）. The plasma activities of PON-1, superoxide dismutase（SOD）, glutathione peroxidase（ GSHPx）, and the contents of malondialdehyde（MDA） were measured in the type 2 diabetes mellitus and 48 normal controls. The analysis of linear correlation was performed to estimate the relationship between these variables. Results The plasma activities of PON-1, GSHPx, SOD were significantly lower in patients with type 2 diabetes meUitus than in the control subjects（ P 〈 0.01）. The plasma MDA levels were significantly higher in patients with type 2 diabetes mellitus than in the control subjects（ P 〈 0.01）. The plasma activities of PON-1, GSHPx, SOD were significantly lower in DMAP than those without microangiopathy（ P 〈 0.05）. The plasma levels of MDA were significantly higher in DMAP than those without microangiopathy（ P 〈 0.05）. The analysis of linear correlation showed PON-1 was positively correlated with GSHPx（ r = 0. 774, P 〈 0.01）, SOD（ r = 0. 801, P 〈 0.01） and negatively correlated with MAD（r = - 0.833, P 〈 0.01）. Conclusion Plasma PON-1 activities were significantly decreased in type 2 diabetes with microangiopathy. Oxidative stress may participate in deteminining PON-1 activity in type 2 diabetes with microangiopathy. The conmlex interaction between oxidative stress and PON-1 may result in accelerated microangiopathy in type 2 diabetes meUitus.