尿液中丁丙诺啡的提取方法

A Study in Extraction Method of Buprenorphine in Urine

目的建立尿液中丁丙诺啡提取方法。方法尿液加pH 7.0缓冲液后,用三氯甲烷进行液相萃取或加pH 10.8缓冲液后,用401有机担体作吸附剂、三氯甲烷作洗脱剂进行固相萃取,GC/NPD法检测。结果尿液中丁丙诺啡液相萃取萃取率可达86.6%以上,固相萃取可达83.0%以上。结论两种提取方法萃取率高,可用于尿液中丁丙诺啡的提取。

Objective The extraction method for separating buprenorphine from urine was described. Methods Buprenorphine was extracted with chloroform at pH7 or with SPE （extracted by organic support 401 at pH10.8, then eluted with chloroform）, finally determined by GC-NPD. Results The extracted yields of the analyte in specimens were 86.6% by solution-phase extraction and 83.0% by solid-phase extraction （SPE） respectively. Conclusion These two methods are simple and accurate for separating buprenorphine from urine.