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DNA甲基化抑制物对雌激素受体β在乳腺癌细胞中表达的影响及意义

Effect and significance of DNA methylation inhibitor on the expression of estrogen receptor-β in breast carcinoma cells
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摘要 目的:研究甲基化抑制物作用于乳腺癌细胞后对其ER-β表达的影响及意义。方法:抽取细胞总RNA,采用半定量逆转录-聚合酶链反应(RT-PCR)检测MCF-7与MDA-MB-231中ER-β的表达状况。在两种细胞中按作用浓度加入甲基化抑制药物5-AZA-CdR,反应一定时间后用RT-PCR的方法测定药物处理后ER-β的表达情况,分析影响及意义。结果:MCF-7的ER-β平均表达水平(0.54±0.44),药物处理后平均表达水平(0.76±0.53),较未经药物处理的细胞明显升高(P<0.05);MDA-MB-231是雌激素受体阴性的细胞株,不表达ER-β,药物处理后ER-β出现表达平均水平为(0.32±0.11),P<0.05。结论:DNA去甲基化可以诱导ER-β表达增强或再表达,对于提高乳腺癌的内分泌治疗疗效和指导新辅助化学治疗有重大意义。 Objective:To investigate the expression of methylation inhibitor.Methods:MDA-MB-231 and MCF-7 ER-β in breast carcinoma ceils before and after exposure to the DNA ceils were treated with 5-AZA-CdR. Total RNA was extracted from the treated and untreated ceils and RT-PCR was used to determine the effect of the treatment on the expression of ER-β.Results:The expression of ER-β in treated MCF-7 ceils was 0.76±0.63 and it was higher than the untreated MCF-7 ceils 0.54±0.44(P〈0.05).The ER(-) MDA-MB-231 ceils were activated by 5-AZA-CdR, The expression of ER-13 in treated MDA-MB-231 was 0.32±0.1 1(P〈0.05).Conelusion:The DNA methylation inhibitor may activate the expression of ER-β in breast carcinoma ceils, it provides a rationale for the use of inhibitor of DNA methylation for the chemotherapy of breast cancer and supports the Endocrine therapy of breast cancer.
出处 《现代医药卫生》 2007年第17期2532-2534,共3页 Journal of Modern Medicine & Health
关键词 乳腺癌细胞 ER-Β DNA甲基化抑制剂 Breast carcinoma ceil Estrogen receptor-β (ER-β) DNA methylation inhibitor
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  • 1Chan MF, Liang G, Jones PA. Relationship between transcription and DNA methylation. Curr Top Microbiol Immunol, 2000, 249:75-86.
  • 2Baylin SB, Herman JG. DNA hypermethylation in tumorigenesis:epigenetics joins genetics. Trends Genet, 2000, 16(4): 168-174.
  • 3RheeI, JairKW, YenRW, etal. CpGmethylation is maintained in human cancer cells lacking DNMT1. Nature, 2000,404(6781):1003-1007.
  • 4Okano M, Xie S, Li E. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells. Nucleic Acids Res, 1998, 26(11) :2536-2540.
  • 5Xie S, Wang Z, Okano M, et al. Cloning, expression and chromosome locations of the human DNMT3 gene family. Gene, 1999, 236(1):87-95.
  • 6Rhee I,BachmanKE,ParkBH,etal. DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Nature, 2002, 416(6880): 552-556.
  • 7Bestor TH. Methylation meets acetylation. Nature, 1998, 393(6683) :311-312.
  • 8Yang X, Yan L, Davidson NE. DNA methylation in breast cancer.Endoc Relat Cancer, 2001, 8(2):115-127.
  • 9LapidusRG, NassSJ, DavidsonNE. The loss of estrogen and progesterone receptor gene expression in human breast cancer. J Mammary Gland Biol Neoplasia, 1998,3(1):85-94.
  • 10Lapidus RG, Nass SJ, Butash KA, et al. Mapping of ER gene CpG island methylation-specific polymerase chain reaction. Cancer Res, 1998,58(12) :2515-2519.

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