胃癌患者外周血CD4^+CD25^+CD127^(lo/-)调节性T细胞的表达及意义

Expression of CD4^+CD25^+CD127^(lo/-) T cells in gastric cancer patients and its significance

目的:探讨胃癌患者外周血中CD4+CD25+CD127lo/-调节性T细胞(regulatory T cells,Treg)的表达及意义。方法:采用流式细胞术检测26例胃癌患者、30例胃溃疡患者和20例健康志愿者外周血中CD4+CD25+CD127lo/-Treg、细胞毒性T细胞(cytotoxic T lympho-cytes,CTL)和NK细胞,采用ELISA法检测血清中γ-干扰素(interferon γ,IFN-γ)的表达水平。结果:胃癌患者外周血中CD4+CD25+CD127lo/-Treg占CD4+T淋巴细胞的百分比为(6.12±2.08)%,高于胃溃疡患者和健康志愿者(P均<0.05)。胃癌患者外周血中CD4+CD25+CD127lo/-Treg水平与CTL、NK细胞及IFN-γ呈负相关。结论:胃癌患者外周血中具有免疫抑制活性的CD4+CD25+CD127lo/-Treg水平较高,对胃癌患者具有肿瘤免疫抑制作用。

Objective ：To evaluate the expressions of CD4^＋CD25^＋CD127^lo T cells in peripheral blood from patients with gastric cancer. Methods ：26 patients with gastric cancer,30 patients with gastric ulcer and 20 healthy volunteers were included in this study. The proportion of CD4^＋CD25^＋CD127^lo- T cells population in CD4^＋ T cells was evaluated by flow cytometric analysis. The proportion of CTL and NK ceils population in lymphocytes was determined by flow cytometric analysis. IFN- γ was measured by ELISA. Results：The population of CD4^＋CD25^＋CD127^lo- T cells in peripheral blood from patients with gastric cancer accounted for （6.12±2.08）% of CD4^＋ T lymphocytes, and was signlficandy higher in comparison with that in patients with gastric ulcer and in healthy volunteers （P〈0.05）. The CD4^＋ CD25^＋CD127^lo- T cells population in gastric ulcer patients was negatively correlated with CTL,NK cells and IFN-γ Conclusion：The CD4^＋CD25^＋CD127^lo- regulatory T cells in peripheral blood of patients with gastric cancer is signlficandy increased in comparison with that in patients with gastric ulcer and in healthy subjects. It may be responsible for immune suppression in gastric cancer patients.