高危型HPV-DNA检测在宫颈病变筛查中的应用价值

Application value of high-risk HPV-DNA test in cervical lesions screening

目的:探讨高危型人乳头瘤病毒(HR-HPV)检测在宫颈病变筛查中的应用价值。方法:应用第二代杂交捕获法(HC-Ⅱ)和液基薄层细胞学(TCT)对839例受检者同时行13种高危型HPV DNA检测和宫颈细胞学检查;HPV阳性和细胞学结果为非典型鳞状细胞(ASCUS)及以上患者行阴道镜下病理活检,分析HPV感染与宫颈上皮内病变的关系。结果:HPV检出率随宫颈病变程度加重呈上升趋势,ASCUS中HPV检测阳性率为75.47%,HPV阳性的ASCUS患者中病理活检47.17%为宫颈上皮内瘤变或更严重病变。结论:HPV检测在宫颈病变筛查中可作为细胞学的辅助手段提高诊断准确性,并可指导HPV感染者的治疗及用于患者的追踪随访。

Objective：To investigate the application value of high-risk human papilloma virus （HR-HPV） DNA test m cervical lesions screening. Methods：839 cases cytological specimens exfoliated from cervix were tested by both the hybrid capture Ⅱ （HC-Ⅱ） assay and the thin prep pap smear fluid-based system （TCT） for detecting 13 types of high-risk HPV DNA. Both patients with positive results of high-risk HPV DNA and pap smear showed atypical squamous cells of undetermined significance （ASCUS） were performed colposcopy and histologic＇,d examination on the cervic＇,d tissue. Results：HPV infection rate increased according to the seriousness degree of cervical lesions. Of the patients with ASCUS and positive results of high-risk HPV DNA ,47.17% of patients were found to have cervical intraepithelial neoplasm （CIN）. Conclusion：HPV DNA test is very important to improve the diagnostic accuracy. Earlier detection of high-risk HPV types can instruct the treatment and trace the infected patients.