神经节苷脂GM1与亚低温联合应用对新生鼠缺氧缺血性脑损伤的保护作用

Neuroprotective effects of ganglioside GM1 and mild hypothermia on hypoxic-ischemic brain damage in neonatal rats

目的探讨神经节苷脂GM1与亚低温对新生大鼠缺氧缺血性脑损伤的保护作用。方法用75只新生7 d的SD大鼠制作缺氧缺血(HIBD)模型,随机分为假手术(A)组、模型(B)组、神经节苷脂GM1(C)组、亚低温(D)组、神经节苷脂与亚低温联合干预(E)组共5组,每组15只。观察不同干预方法对新生大鼠缺氧缺血后24h脑含水量、神经细胞死亡率及30d后学习和记忆能力的影响。结果B组脑含水量、神经细胞死亡率显著高于A组,学习、记忆能力显著下降(P<0.05),各干预组脑含水量、神经细胞死亡率下降,学习、记忆能力显著改善(P<0.05),而以E组改变更明显(P<0.05)。结论神经节苷脂GM1和(或)亚低温均可显著减轻HI后脑损伤,联合干预保护作用更佳。

Objective on hypoxic-ischemic brain To investigate the protective effects of ganglioside GM1 and/or mild hypothermia damage in neonatal rats. Methods Seventy-five neonate rats （subjected to unilateral left carotid artery ligation and given 8% oxygen for 2 h） were randomly allocated to five groups：sham operation group （ A）, model group （ B）, GM1 group （ C ）, hypothermia group （ D ）, GM1 and hypothermia group （ E ）. Changes of brain edema and percentage of dead neurons（HDNP） at 24 h and behaviors of learning and memory at 30 d after HI insult were investigated. Results Brain edema and HDNP were significantly increased in group B compared with group A,whereas behaviors of learning and memory in group B were reduced significantly. Brain edema, HDNP and behaviors were improved in group C, D and E （ P 〈 0.05 ）, the most obvious changes was observed in group E （P 〈0.05 ）. Conclusion Ganglioside GM1 and/or mild hypothermia have protective effects on HIBD,especially when ganglioside GM1 and mild hypothermia are used simultaneously.