Mo(Ⅵ)-邻硝基苯基荧光酮配合物光谱探针测定蛋白质

Determination of micro amount of protein with o-Nitrophenylfluorone-Molybdenum(Ⅵ) as a spectroscopic probe

研究了Mo（Ⅵ）-邻硝基苯基荧光酮（o-NPF）配合物与蛋白质的相互作用及光谱性质。提出了以Mo（Ⅵ）-邻硝基苯基荧光酮配合物作为光谱探针测定微量蛋白质的方法。结果表明，在pH为2.2的HAc-NaAc缓冲介质中，乳化剂OP存在下，o-NPF-Mo（Ⅵ）在40℃加热10 min后与蛋白质形成稳定的复合物，最大吸收波长红移至587 nm。蛋白质质量浓度在0-10 mg/L范围内符合比尔定律，复合物的表观摩尔吸光系数为3.53×10^6L·mol^-1·cm^-1。该方法具有很高的灵敏度和选择性，可直接用于人尿、新生牛血清中蛋白质的测定，回收率分别为93%和99%。

The interaction and spectral charactenstics of o-Nitmphenylfluomne-Molybdenum（ Ⅵ ） and protein was studied. A novel method for the determination of protein using o-NPF-Mo（ Ⅵ ） complex as a spectroscopic probe was developed. In the buffer medium of HCl-NaAc at pH 2.2 and in the presence of p-octylpolyethylenther（OP）, o-NPF-Mo （ Ⅵ） complex combines protein at 40 ℃ for 10 min to form a stable compound, which results in change of absorption spectrum of o-NPF-Mo（ Ⅵ ） complex and increase of the absorption peak value at 587 nm. Beer＇s law was obeyed in the range of 0 - 10 mg/L for protein. The molar absorptivity of the complex at 587 nm was 3.53 - 10^6 L/（mol·cm）. The method has high sensitivity as well as high selectivity. It could be used to determine protein in human urine, binvine serum and the recoveries were between 93 % and 99%.