青钱柳中多糖的测定

Determination of contents of polysaccharides in Cyclocarya paliurus(βatal) Iljinsk

建立了一种测定青钱柳中多糖的方法。青钱柳样经80%乙醇回流除去单糖、双糖、低聚糖、多酚、氨基酸等杂质后用水提取,得青钱柳多糖,其含量采用蒽酮-硫酸法比色测定,用精制青钱柳多糖测得青钱柳多糖对葡萄糖的换算因子。采用该方法测定简便可行,供试液在8 h内显色稳定,重现性较好,平均回收率为100.8%,RSD为1.9%(n=3)。所测江西修水青钱柳中多糖含量为4.39%。

A simple and accurate method for the determination of the contents of polysaccharides in Cyclocarya paliurus （βatal） Iljinsk was eatablished. Polysaccharides in Cyclocarya paliurus （βatal） Iljinsk samples were extracted with water, after the interference components such as monosaccharide, disaccharide, oligosaccharide, polyphenol and amino acids in the samples were removed by back-flow with 80% ethanol. The contents of the polysaccharides were determined by anthrone-sulfuric acid colorimetry. To minimize the error of determination, a corrected factor was used. The results obtained showed that the method used in this paper was simple and available, and the color of the treated samples was stable within 8 h. The average value of the recovery for the determination of the polysaccharides was 100.8% with RSD of 1.9% （ n = 3）. The contents of polysaccharides in the Cyclocaryapaliurus （βatal） Iljinsk sampies of Xiu-Shui county determined are 4.387%.