CIK治疗肝硬化患者抗病毒疗效及对淋巴细胞和DC的影响

Antiviral effect and influence on lymphocyte and dendritic cell of autologous cytokine-in-duced killer cells in patients with liver cirrhosis caused by chornic HBV infection

目的:观察自体细胞因子诱导杀伤细胞(CIK)治疗对于慢性HBV感染所致的肝硬化患者淋巴细胞和树突细胞的影响以及其抗病毒疗效。方法:33例肝硬化患者给予CIK细胞治疗,在治疗前后检测患者淋巴细胞亚群和mDC以及pDC的数量、病毒学标志以及其他不良反应。结果:CIK治疗后,12例患者HBVDNA阴转,总有效率达51.5%。14例HBeAg阳性患者中有10例阴转,5例患者出现HBeAb转换。在治疗无效病例仅CD8+T淋巴细胞、NKT细胞较治疗前有明显增高,而有效病例则表现出CD3+、CD4+、CD8+T淋巴细胞以及NKT细胞均较治疗前明显增高。且有效病例mDC、pDC的数量较治疗前有明显增高。结论:CIK细胞治疗对于慢性HBV感染所致的肝硬化患者有一定的抗病毒疗效。其可诱导体内多种免疫活性细胞增高。

Objective：to observe the antiviral effect and influence on lymphocyte and dendritic cell （DC） of autologous cytokine-induced killer cells （CIK） in patient with liver cirrhosis caused by chornic HBV infection. Methods：33 patients with liver cirrhosis caused by HBV chronic infection were treated with CIK. The quantity of lymphocyte subset, mDC and pDC were detected by flow cytometry before and after CIK treatment. The markers of virology and the adverse reaction were also observed. Results：Mter treatment, HBV DNA in 12 patients couldn＇t be detected. The total effective power was 51.5%. HBeAg in 10 patients turned to negative in 14 patients who were positive in HBeAg, among them 5 patients appeared HBeAb conversion. CD8^＋ T lymphocyte, NKT cell increased in ineffective patients after CIK transfused back, but all of the CD3 ^＋ , CIM^＋ , CD8^＋ T lymphocyte and NKT cell increased in effective patients. Besides, the quantity of mDC, pDC also increased than pre treatment in effective patients. Condusion：CIK has some antivirol effect in patients with liver cirrhosis caused by chronic HBV infection. Immune cells increase after CIK treatment.