实时荧光定量PCR检测HBV DNA与ELISA检测HBV M结果的关系

RELATIONSHIPS BETWEEN DETECTION OF HBV DNA BY REAL-TIME FLUORESCENT QUANTITATIVE PCR AND DETECTION OF HBV M BY ELISA

目的:探讨实时荧光定量PCR(FQ-pCR)检测HBV DNA与ELISA检测HBV M结果的关系。方法:采用实时FQ-PCR和ELISA方法分别对240例乙肝患者进行HBV DNA定量测定和HBV M检测。结果:240例HBsAg阳性血清中HBV DNA阳性率为74.58%。HbsAg、HbeAg、HBcAb和HbsAg、HbeAg阳性组的HBV DNA含量及HBV DNA阳性率均明显高于HbsAg、HbeAb、HBcAb和HbsAg、HBcAb组(P<0.05,P<0.01)。122例HBeAg阳性的血清中97.54%的HBV DNA阳性;118例HBeAg阴性的血清中有50.85%HBV DNA阳性。结论:实时FQ-PCR检测HBV DNA准确灵敏,是HBV感染及复制的直接证据。HBV M和HBV DNA的检测各有其独特的临床检测意义,在乙型肝炎的诊断治疗中均具有重要的临床价值。

Objective：To investigate the relationships between HBV DNA detected by real-time fluorescent quantitative PCR （FQ-PCR） and detection of HBV M by ELISA.Methods：HBV DNA and HBV M in 240 HB patients blood were respectively detected by real-time FQ-PCR and ELISA.Resuits：The total HBV DNA positive ratio in serum was 74.58%.The HBV DNA content and positive ratio of HBsAg,HBeAg,HbcAb and HBsAg, HbeAg group were all significantly higher than that of the HBsAg,HBeAb,HbcAb and HBsAg,HbcAb group （P 〈 0.05, P 〈 0.01）.The positive ratio of HBV DNA was 97.54% in 122 HBeAg positive serum;while the positive ratio of HBV DNA was 50.85% in 118 HBeAg negative serum.Conclusions：The detection of HBV DNA by real-time FQ-PCR is accurate and sensitive, it can provide direct evidence for HBV infection and replication.The detection of HBV DAN and HBV M has their own unique clinical significance and all have important clinical value in diagnosis and therapy of HBV hepatitis.