夹层杯集菌离心涂片法检测痰抗酸杆菌对肺结核诊断价值的研究

The value of detection mycobacterium tuberculosis by centrifuging the liquored sputa and collecting bacteria in diagnosis of pulmonary tuberculosis

目的:探讨夹层杯集菌离心涂片法(夹层杯法)检测痰抗酸杆菌对肺结核的诊断价值。方法:采用夹层杯法使痰液充分液化,利于抗酸杆菌充分集中沉淀于特制的夹层杯底部的菌膜上,从而提高检出率。结果:该方法检测抗酸杆菌的阳性率为31.6%,分别明显高于本研究所传统涂片法的15.9%,培养法的11.9%,分别具有非常显著的临床意义(P<0.005)。结论:该方法简单、省时、阳性率高,并且便于进行质量控制,可能是一种具有推广价值的检测方法。

Objective：Now positive outcome in sputa swear and cutural has strong diagnosis value in pulmonary tuberculosis, but the positive rate is low,the time of sputa cutural is long, finding out a new sputa checking way to improve the diagnosis of pulmonary tuberculosis.Methods： The testing box, Peng＇ s bug and QT- 12 aatomatic centrifuge were provided by TianQi - Tech new medical systems CO,LTD. The sputa were liquored completely and high - speed centri- fuged in order to make the Mycobactefium tubexculosis sink and collect on a special membrane, detecting the bacteria an the membrane, the positive results were obviously improved by the way.Results：The positive rate of this new way is 31.6%, it is significently higher than 15.9% of sputa swear and 11.9% of sputa cultural. There was a significendy difference（ P 〈 0.005）in positive rate among them. Conclusion： The new checking way is simple, sparing time, high positive rate and speciality, easily queality control, can be used as a valuable detecting way of mycobactefium tuberculosis in sputa in clinical.